摘要： 目的 探讨微RNA-217 （miR-217）调节矮小相关转录因子2 （Runx2）和骨桥蛋白（OPN）分子在小鼠胫骨假体周围骨溶解（PPOL）中的作用机制。方法 建立钛颗粒诱导的小鼠胫骨PPOL模型，外源性miR-217 mimics作用PPOL小鼠1~2周。采用micro-CT检测PPOL小鼠胫骨微结构（骨密度、骨矿物质含量及骨体积比）。采用实时荧光定量PCR （RT-qPCR）和Western blotting检测小鼠胫骨PPOL骨组织内Runx2和OPN mRNA和蛋白表达情况。结果 去内毒素的钛颗粒成功诱导小鼠胫骨骨髓腔PPOL效应，外源性miR-217 mimics参与PPOL作用，在第1周和第2周时，PPOL小鼠胫骨的骨密度、骨矿物质含量及骨骼体积比均显著升高（P<0.05）；miR-217 mimics可呈时间依赖性促进小鼠胫骨PPOL骨组织内Runx2和OPN mRNA和蛋白的表达（P<0.05）。结论 miR-217可促进PPOL小鼠胫骨骨组织内Runx2和OPN mRNA和蛋白表达，增加PPOL小鼠胫骨的骨密度及骨矿物质含量，改善PPOL小鼠的骨溶解病理效应。

miR-217调节Runx2/OPN在钛颗粒诱导小鼠胫骨骨溶解中的作用

张晓倩, 王鹏皓, 郭磊

中国医科大学附属第一医院骨科, 沈阳 110001

收稿日期:2022-05-09出版日期:2023-01-30发布日期:2023-02-01
通讯作者:郭磊E-mail:guolei@cmu.edu.cn
作者简介:张晓倩（1986-），女，护师，本科.
基金资助:国家自然科学基金（81971322）


关键词: 假体周围骨溶解, 钛微粒, miR-217, 矮小相关转录因子2, 骨桥蛋白, 骨密度
Abstract: Objective To investigate the mechanism of microRNA-217 (miR-217) regulating the expression of runt stature-related transcription factor 2 (Runx2) and osteopontin (OPN) in titanium particle-induced periprosthetic osteolysis (PPOL) in mouse tibial bone tissue. Methods A mouse model of tibial PPOL was established;exogenous miR-217 mimics were disposed on PPOL for 1 to 2 weeks. The tibial microstructure of mice with PPOL including bone density, mineral content, and volume ratio was detected via micro-CT. The mRNA and protein expressions of Runx2 and OPN were detected via quantitative real-time PCR and western blotting of the bone tissue. Results Endotoxin removing-titanium particles induced the PPOL effect in the tibial bone marrow cavity of mice. Exogenous miR-217 mimics significantly increased bone mineral density, mineral content, and volume ratio of the tibia of PPOL mice at 1 and 2 weeks (P<0.05) and also significantly enhanced the mRNA and protein expression of Runx2 and OPN (P<0.05). This enhancement was a time-dependent phenomenon. Conclusion miR-217 can positively promote the mRNA and protein expression of Runx2 and OPN in the tibial bone tissues of PPOL mice, significantly enhance bone mineral density and content of the tibia of PPOL mice, and effectively improve the osteolytic pathological effects in PPOL mice.
Key words: periprosthetic osteolysis, titanium particles, microRNA-217, runt-related transcription factor 2, osteopontin, bone mineral density
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