摘要： 目的 探讨PAX1、ZNF582甲基化检测及人乳头瘤病毒（HPV）定量检测、HPV16/18分型检测、液基细胞学检测在HPV阳性患者分流中的效能。方法 选取HPV检测阳性患者行PAX1及ZNF582甲基化检测、HPV定量检测、液基细胞学检测。根据宫颈活检病理结果将患者分为CIN1-组、CIN2+组、CIN2-组、CIN3+组。探讨不同检测方法对HPV阳性患者的分流效能以及对CIN2+及CIN3+的诊断效能。结果 PAX1、ZNF582甲基化检测较细胞学检测具有更高的特异度以及阳性预测值（PPV）。PAX1、ZNF582甲基化检测、HPV16/18定量检测在CIN1-组与CIN2+组间、CIN2-组与CIN3+组间的差异均有统计学意义（P<0.05）。PAX1、ZNF582甲基化检测与宫颈活检病理之间存在相关性。结论 在HPV检测阳性患者中，PAX1、ZNF582甲基化检测、HPV16/18定量检测具有一定的分流效能。

不同检验方法在HPV阳性患者分流中的效能

张诗敏, 王敏

中国医科大学附属盛京医院妇产科, 沈阳 110004

收稿日期:2022-11-07出版日期:2023-01-30发布日期:2023-02-01
通讯作者:王敏E-mail:wm21st@126.com
作者简介:张诗敏（1997-），女，硕士研究生.
基金资助:辽宁省教育厅科学研究经费项目（ZF2019017）；沈阳市科学技术计划（19-112-4-021）；盛京医院科研项目（M0067）；盛京自由研究者项目（201705）


关键词: 宫颈癌筛查, 人乳头瘤病毒, 甲基化, 分流
Abstract: Objective To explore the performance of PAX1 and ZNF582 methylation detection, human papilloma virus (HPV) quantitative detection, HPV16/18 detection, and cytology in the triage of HPV-positive patients. Methods PAX1 and ZNF582 methylation detection, HPV quantitative detection, and cytological detection were performed in patients with positive HPV detection. The patients were divided into CIN1-, CIN2+, CIN2-, and CIN3+ groups according to the pathological results of a cervical biopsy. The efficacy of the different tests in the triage of HPV-positive patients and the diagnostic efficacy of CIN2+ and CIN3+ were investigated. Results PAX1 and ZNF582 methylation detection had higher specificities and positive predictive values than cytology. The difference between the CIN1-group and CIN2+ groups and between the CIN2- and CIN3+ groups in PAX1 and ZNF582 methylation detection and HPV16/18 quantitative detection were statistically significant (all P<0.05). There was correlation between PAX1 and ZNF582 methylation detection and cervical biopsy pathology. Conclusion PAX1 and ZNF582 methylation detection and HPV16/18 quantitative detection show triage efficiency in HPV-positive patients.
Key words: cervical cancer screening, human papilloma virus, methylation, triage
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