摘要： 目的 探讨硒对高糖诱导的大鼠腹膜间皮细胞上皮-间质转化（EMT）的影响。方法 将40只清洁级SD雄性大鼠随机分为对照组（NC组）、高糖组（HG组）、低硒组（HG+L-Se组）、高硒组（HG+H-Se组），每组10只，分别腹腔注射无菌生理盐水、4.25%葡萄糖腹膜透析液、4.25%葡萄糖腹膜透析液和3 mg/kg亚硒酸钠、4.25%葡萄糖腹膜透析液和6 mg/kg亚硒酸钠。腹腔注射28 d后，量取腹腔流出液，计算超滤量。HE染色检测大鼠腹膜组织形态学改变，免疫组织化学染色检测大鼠腹膜组织EMT情况，分光光度法检测大鼠血清丙二醛（MDA）、谷胱甘肽（GSH）含量和总超氧化物岐化酶（T-SOD）活性。结果 与NC组比较，HG组大鼠腹膜超滤量明显减少，腹膜厚度明显增加（均P<0.05）；E-cadherin蛋白表达明显降低，vimentin蛋白表达明显增加（均P<0.05）；血清MDA含量明显增加，GSH含量、T-SOD活性明显降低（均P<0.05）。与HG组比较，HG+L-Se组和HG+H-Se组大鼠腹膜超滤量增加，腹膜厚度减少；E-cadherin蛋白表达增加，vimentin蛋白表达降低；MDA含量降低，血清GSH含量、T-SOD活性升高。且HG+L-Se组与HG组比较，上述指标的差异均无统计学意义（均P>0.05）；HG+H-Se组与HG组比较，上述指标的差异均有统计学意义（均P<0.05）。结论 硒可部分逆转高糖诱导的大鼠腹膜形态和功能的改变，通过抑制氧化应激，延缓大鼠腹膜间皮细胞出现EMT。

硒对高糖诱导的大鼠腹膜透析相关腹膜纤维化的影响

高利丽¹, 蔡宏宇², 刘彤崴¹

1. 锦州医科大学附属第一医院肾内科, 锦州 121001;
2. 锦州医科大学附属第一医院心内科, 锦州 121001

收稿日期:2022-04-02出版日期:2023-01-30发布日期:2023-02-01
通讯作者:刘彤崴E-mail:13504069262@163.com
作者简介:高利丽（1982-），女，主治医师，博士.
基金资助:辽宁省自然科学基金（2021-BS-269）


关键词: 腹膜间皮细胞, 高糖, 硒, 氧化应激, 上皮-间质转化
Abstract: Objective To observe the effects of selenium on high glucose-induced epithelial-mesenchymal transition (EMT) in rat peritoneal mesenchymal cells. Methods Forty male SD rats were randomly divided into the following groups:control (NC), high glucose (HG), high glucose + low-dose selenium (HG+L-Se), and high glucose + high-dose selenium (HG+H-Se) groups (n=10 per group). After 28 days of intraperitoneal injections, the peritoneal effluent was measured and the amount of ultrafiltration was calculated. The morphological changes in peritoneal tissues were detected by HE staining. The EMT in peritoneal tissues was detected by immunohistochemical staining. The serum levels of malondialdehyde (MDA), glutathione (GSH), and total superoxide dismutase (T-SOD) were detected by spectrophotometry. Results In comparison to the NC group, the HG group had the following results:the peritoneal ultrafiltration function significantly decreased and the peritoneal thickness significantly increased (both P<0.05), the expression of E-cadherin significantly decreased and the expression of vimentin significantly increased (both P<0.05), the serum level of MDA significantly increased, and the serum levels of GSH and T-SOD significantly decreased (all P<0.05). In comparison to the HG group, the HG+L-Se and HG+H-Se groups had the following results:the peritoneal ultrafiltration function increased and the peritoneal thickness reduced, the expression of E-cadherin increased and the expression of vimentin decreased, the serum level of MDA decreased, and the serum levels of GSH and T-SOD increased. Significant differences were found in the above indicators between the HG+H-Se and HG groups (all P<0.05), while no significant differences were found between the HG+L-Se and HG groups (all P>0.05). Conclusion Selenium can partially reverse the changes in rat peritoneal morphology and function that are caused by high glucose, and the EMT in rat peritoneal mesothelial cells can be delayed by inhibiting oxidative stress.
Key words: peritoneal mesenchymal cell, high glucose, selenium, oxidative stress, epithelial-mesenchymal transition
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