摘要： 目的 探讨前列腺六跨膜上皮抗原3 （STEAP3）在脑缺血再灌注（I/R）损伤沙鼠中的表达及意义。方法 将48只沙鼠随机分为假手术组（Sham组）、I/R模型组（I/R组）、I/R模型+STEAP3 siRNA组（siRNA组）和I/R模型+scramble siRNA组（Control组），每组12只。进行沙鼠神经功能评分。Nissl染色观察海马CA1区神经元。Fe²⁺试剂盒检测海马组织Fe²⁺水平。Western blotting检测海马组织中STEAP3、H2AX、4-HNE、GPX4蛋白的表达。应用丙二醛（MDA）试剂盒检测海马组织中MDA含量。结果 I/R组沙鼠神经功能学评分、STEAP3、H2AX、4-HNE蛋白及MDA和Fe²⁺水平明显高于Sham组（P<0.05），而神经元数量和GPX4蛋白表达明显低于Sham组（P<0.01）；与I/R组比较，siRNA组沙鼠神经功能学评分、STEAP3、H2AX、4-HNE蛋白及MDA和Fe²⁺水平明显降低（P<0.05），神经元数量和GPX4蛋白表达明显增多（P<0.05）。结论 STEAP3在沙鼠脑I/R损伤中高表达，通过介导铁死亡影响脑I/R损伤的发生和发展，抑制其表达对脑I/R损伤具有保护作用。

STEAP3在脑缺血再灌注损伤沙鼠中的表达及意义

李晓蕾¹, 李忠华², 王鹏³

1. 贵州医科大学第三附属医院实验中心, 贵州 都匀 558000;
2. 贵州医科大学第三附属医院神经外科, 贵州 都匀 558000;
3. 哈尔滨医科大学大庆校区基础医学院生理学教研室, 哈尔滨 大庆 163319

收稿日期:2022-06-20出版日期:2023-01-30发布日期:2023-02-01
通讯作者:王鹏E-mail:1905901711@qq.com
作者简介:李晓蕾（1978-），女，副教授，博士.
基金资助:贵州省科技计划项目（黔科合基础［2019］1281）；黑龙江省博士后资助经费（LBH-Z18276）


关键词: 缺血再灌注损伤, 脑, 前列腺六跨膜上皮抗原3, 铁死亡, 沙鼠
Abstract: Objective To explore the expression of six-transmembrane epithelial antigen of prostate 3 (STEAP3) and its significance in gerbils with cerebral ischemia-reperfusion (I/R) injury. Methods Forty-eight gerbils were randomly divided into four groups:a sham group, an I/R model group (I/R group), an I/R model+STEAP3 siRNA group (siRNA group), and an I/R model+scramble siRNA group (control group), with n=12 per group. Neurological function was scored. Nissl staining was performed to observe the neurons in the hippocampus. The expression of STEAP3, H2AX, 4-HNE, and GPX4 in the hippocampus was measured using Western blotting. The levels of malondialdehyde (MDA) and Fe²⁺ were assessed. Results The neurological function scores, the expression of STEAP3, H2AX, and 4-HNE, and the levels of MDA and Fe²⁺ were significantly higher in the I/R group (P<0.05) than in the sham group, and the expression of GPX4 and the number of normal neurons were significantly lower (P<0.01). The neurobehavioral scores, the expression of STEAP3, H2AX, and 4-HNE, and the levels of MDA and Fe²⁺ were significantly lower in the siRNA group (P<0.05) than in the I/R group, and the expression of GPX4 and the number of normal neurons were significantly higher (P<0.05). Conclusion Increased expression of STEAP3 was found in gerbils with cerebral I/R injury, which may affect the occurrence and development of cerebral I/R injury by mediating ferroptosis. Knockout of STEAP3 can protect the brain from I/R injury.
Key words: ischemia-reperfusion injury, brain, STEAP3, ferroptosis, gerbils
PDF全文下载地址:

https://journal.cmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=3144