摘要： 目的 探讨鱼腥草素钠（SH）对中性粒细胞哮喘（NA）小鼠炎性细胞因子肿瘤坏死因子-α （TNF-α ）、白细胞介素-1β （IL-1β ）及黏蛋白5AC（MUC5AC）的影响。方法 将40只小鼠随机分为NC组、NA组、SH组、地塞米松（DXM）组、SH+DXM组。检测各组小鼠气道阻力；计数支气管肺泡灌洗液（BALF）中白细胞总数；瑞氏吉姆萨染色分类计数白细胞；HE、PAS、Masson染色观察肺组织及气道周围炎症细胞分布、黏液分泌、纤维化程度；免疫组化（IHC）法检测肺组织中MUC5AC的表达差异，实时荧光定量PCR（qRT-PCR）检测肺组织中MUC5AC mRNA含量，Western blotting检测肺组织中TNF-α、IL-1β、p-AKT/AKT的蛋白表达。结果 与NA组小鼠相比，SH组、DXM组、SH+DXM组小鼠气道阻力、BALF中白细胞总数及中性粒细胞计数均减少，肺组织及气道周围炎症、黏液分泌、纤维化减轻，肺组织及气道上皮细胞MUC5AC蛋白表达量减少，肺组织MUC5AC mRNA含量及TNF-α、IL-1β、p-AKT/AKT蛋白表达量减少（均P < 0.05）。结论 SH可通过抑制AKT磷酸化减少炎性细胞因子及黏蛋白表达，缓解NA症状。

鱼腥草素钠对中性粒细胞哮喘小鼠炎性细胞因子和黏蛋白表达的影响

黄晨凤, 李淼

中国医科大学附属盛京医院小儿呼吸内科, 沈阳 110004

收稿日期:2021-12-08发布日期:2022-11-09
通讯作者:李淼E-mail:Lmpediatrician@126.com
作者简介:黄晨凤（1996-），女，硕士研究生.
基金资助:国家自然科学基金青年基金（81400016）


关键词: 鱼腥草素钠, 中性粒细胞哮喘, 肿瘤坏死因子-α, 白细胞介素-1β, 黏蛋白5AC, 丝氨酸/苏氨酸蛋白激酶磷酸化
Abstract: Objective To investigate the effect of sodium houttuyfonate（SH）on the levels of inflammatory factors，such as tumor necrosis factor（TNF）-α，interleukin（IL）-1β ，and mucin 5 subtype AC（MUC5AC）in mice with neutrophilic asthma（NA）.Methods Forty BALB/c mice were randomly divided into NC，NA，SH，dexamethasone（DXM），and SH+DXM groups. Airway resistance was determined for mice in each group and the total number of leukocytes in the bronchoalveolar lavage fluid（BALF）was determined. Wright-Giemsa staining was performed to classify and count leukocytes and hematoxylin and eosin，periodic acid-Schiff，and Masson’s trichrome staining were performed to observe the distribution of inflammatory cells，mucus secretion in lung tissue and around the airways，and the degree of fibrosis，respectively. Immunohistochemistry was performed to detect differences in the expression levels of MUC5AC in lung tissue and real-time polymerase chain reaction was used to determine MUC5AC mRNA levels in lung tissue. Western blotting was used to detect differences in TNF-α，IL-1β ，and phosphorylated（p）-AKT/AKT protein levels in lung tissue.Results Compared with the NA group，airway resistance，and the total number of leukocytes and neutrophils in BALF were lower in the SH，DXM，and SH+DXM groups（P < 0.05）. Inflammation，mucus secretion，and fibrosis in the lung tissue and around the airways were reduced and MUC5AC protein expression levels in lung tissue and airway epithelial cells decreased（P < 0.05）. MUC5AC mRNA levels and TNF-α，IL-1β ，and p-AKT/AKT protein levels in lung tissue decreased（P < 0.05）.Conclusion SH may reduce the expression levels of inflammatory factors and mucin by inhibiting the phosphorylation of AKT，thus alleviating the symptoms of NA.
Key words: sodium houttuyfonate, neutrophilic asthma, tumor necrosis factor-α, interleukin-1β, mucin 5 subtype AC, serine/threonine protein kinase phosphorylation
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