摘要： 目的 基于多数据库探讨FAM110A在乳腺癌中的表达及临床意义。方法 通过Oncomine数据库和GEPIA网站对FAM110A mRNA表达进行泛癌分析。采用Oncomine数据库和UALCAN数据库对FAM110A在乳腺癌中的表达进行分析。采用GEPIA分析FAM110A表达水平与患者生存预后的关系。基于HPA数据库探讨FAM110A基因在肿瘤组织中的表达情况及定位。采用cBioportal和STRING数据库分析互作蛋白构建共表达分子调控网络。采用DAVID网站对FAM110A的共表达分子进行基因本体论(GO)及京都基因和基因组(KEGG)富集分析。结果 在乳腺癌中FAM110A的表达显著上升(P < 0.05),且与临床分期相关(F=2.84,P < 0.05)。FAM110A高表达的乳腺癌患者预后不良(P < 0.05)。GO和KEGG富集分析结果显示,FAM110A的共表达基因主要参与DNA转录调控和泛素介导的蛋白质水解等通路。结论 FAM110A在乳腺癌中的表达高于正常组织,高表达患者预后不良,FAM110A的表达与乳腺癌肿瘤分化水平有关。

基于生物信息学分析FAM110A在乳腺癌中的表达及临床意义

马小雯^1,2,3,4, 杨巍⁵, 郝靓^1,2,3

1. 中国医科大学法医学院化学教研室, 沈阳 110122;
2. 辽宁省法医学生物证据重点实验室, 沈阳 110122;
3. 中国医科大学司法鉴定中心, 沈阳 110122;
4. 中国医科大学临床二系, 沈阳 110122;
5. 辽宁省健康产业集团本钢总医院感染部, 辽宁 本溪 117000

收稿日期:2021-10-19出版日期:2022-08-30发布日期:2022-07-19
通讯作者:郝靓E-mail:lhao@cmu.edu.cn
作者简介:马小雯（2002-），女，本科在读.
基金资助:国家自然科学基金青年基金（82103693）；辽宁省教育厅科学研究经费项目面上项目（LJKZ0787）；2021年中国医科大学大学生创新创业训练计划（X202110159011）


关键词: 乳腺癌, FAM110A, 生物信息学
Abstract: Objective To analyze the expression difference and clinical significance of FAM110A in breast cancer based on several databases. Methods We used Oncomine and GEPIA to explore the mRNA expression of FAM110A gene in several cancers. We chose the Oncomine and UALCAN databases to analyze the expression of FAM110A gene in breast cancer. The GEPIA was employed to discuss the relevance between FAM110A expression and survival prognosis by investigating the expression and localization of the FAM110A gene in tumor tissues based on the HPA database. The cBioportal and STRING databases were used to search for co-expressed genes of FAM110A in human breast cancer tissues and to construct a co-expressed gene network. We adopted the DAVID platform to analyze the co-expressed genes of FAM110A with GO and KEGG. Results The expression of FAM110A in breast cancer was higher than that in normal tissues(P < 0.05), and its expression level was related to clinical stage(F=2.84,P < 0.05). The prognosis of breast cancer patients who highly expressed FAM110A were poor(P < 0.05). The GO cluster and KEGG pathway enrichment analysis showed that the FAM110A co-expressed genes were mainly involved in regulating DNA template transcription and ubiquitin mediated protein hydrolysis pathway, etc. Conclusion The expression of FAM110A in breast cancer is higher than that in normal tissues, and the prognosis of patients with high FAM110A expression is poor. This suggests that FAM110A expression is associated with a degree of tumor differentiation.
Key words: breast cancer, FAM110A, bioinformatics
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