摘要： 目的 探讨同源异型盒基因A10(HOXA10)对非小细胞肺癌(NSCLC)细胞安罗替尼敏感性的影响。方法 采用实时定量PCR检测HOXA10在NSCLC细胞中的表达。应用小干扰RNA在A549和PC-9细胞中敲低HOXA10表达,并用不同浓度安罗替尼处理细胞。Western blotting检测HOXA10蛋白表达；CCK-8法检测细胞增殖活性,以明确安罗替尼的细胞毒性,并计算半数抑制浓度(IC₅₀)；克隆形成实验检测细胞增殖；Transwell实验检测细胞迁移和侵袭能力；流式细胞术检测细胞凋亡。结果 HOXA10在NSCLC细胞中呈高表达；敲低HOXA10能够降低安罗替尼在A549和PC-9细胞中的IC₅₀；敲低HOXA10能够增强安罗替尼对肿瘤细胞增殖、迁移和侵袭的抑制能力；敲低HOXA10能够促进安罗替尼诱导的细胞凋亡。结论 敲低HOXA10能够增加NSCLC细胞对安罗替尼的敏感性。

HOXA10基因敲低对非小细胞肺癌安罗替尼敏感性的影响

李俞羲¹, 路遥², 田野²

1. 中国医科大学附属第四医院麻醉科, 沈阳 110032;
2. 中国医科大学附属第四医院胸外科, 沈阳 110032

收稿日期:2021-10-22出版日期:2022-06-30发布日期:2022-06-09
通讯作者:田野E-mail:Ono1180@gmail.com
作者简介:李俞羲(1994-),女,医师,硕士.
基金资助:辽宁省自然科学基金(JCZR2020016)


关键词: 同源异型盒基因A10, 安罗替尼, 非小细胞肺癌, 敏感性
Abstract: Objective To investigate the effect of homeobox A10(HOXA10)on the sensitivity of non-small cell lung cancer(NSCLC) cell lines to anlotinib. Methods Quantitative real-time PCR was used to determine the expression of HOXA10 mRNA in NSCLC cells. A549 and PC-9 cells were transfected with si-HOXA10,and the expression of HOXA10 protein was detected using Western blotting. The transfected cells were then treated with anlotinib. Cell viability and half-maximal inhibitory concentration(IC₅₀)were determined using the CCK-8 assay. Colony-forming ability was assessed using a colony formation assay. Cell migration and invasion were determined using Transwell assay. Annexin-V/PI staining and flow cytometry were performed to detect cell apoptosis. Results HOXA10 was remarkably upregulated in NSCLC cells. HOXA10 knockdown significantly decreased the IC₅₀ values of anlotinib in both A549 and PC-9 cells. HOXA10 knockdown enhanced the inhibitory effect of anlotinib on colony formation,cell migration,and cell invasion. HOXA10 knockdown promoted anlotinib-induced apoptosis. Conclusion HOXA10 knockdown increases the sensitivity of NSCLC cells to anlotinib.
Key words: homeobox A10, anlotinib, non-small cell lung cancer, sensitivity
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