摘要： 目的探讨大黄酸对喉癌Hep-2细胞增殖、侵袭和迁移的作用及其与Wnt/β-catenin信号通路的关系。方法利用低、中、高浓度（6、12、24 μmol/L）大黄酸处理喉癌Hep-2细胞，通过MTT法、划痕实验、Transwell法分别检测细胞的增殖、迁移和侵袭能力，应用实时荧光定量PCR和免疫印迹法检测β-catenin、Dvl2、GSK-3β和p-GSK-3β在Hep-2细胞中的表达水平。结果随着大黄酸浓度的增大，喉癌细胞的增殖、迁移和侵袭能力显著减弱（P<0.05）。大黄酸可降低喉癌细胞Dvl2、p-GSK-3β和β-catenin蛋白的表达水平（P<0.05），增加GSK-3β蛋白的表达（P<0.05）。使用SKL2001激活Wnt/β-catenin信号通路，能够逆转大黄酸对Hep-2细胞迁移和侵袭的抑制作用（P<0.05）；使用XAV939抑制Wnt/β-catenin信号通路，能够降低Dvl2、p-GSK-3β和β-catenin的蛋白表达水平（P<0.05），进一步加强大黄酸对Hep-2细胞迁移的抑制作用（P<0.05）。结论大黄酸通过抑制Wnt/β-catenin信号通路，有效抑制喉癌细胞的增殖、侵袭和迁移。

大黄酸通过Wnt/β-catenin信号通路抑制喉癌细胞的增殖、迁移和侵袭

孙倩, 刘盛力, 马钰, 杜莉

中国医科大学附属第四医院耳鼻咽喉科, 沈阳 110032

收稿日期:2021-12-24出版日期:2022-05-30发布日期:2022-05-28
通讯作者:杜莉
作者简介:孙倩(1989-),女,医师,硕士研究生.
基金资助:辽宁省自然科学基金（20170541000）


关键词: 喉癌细胞, 大黄酸, 增殖, 侵袭, Wnt/β-catenin信号通路
Abstract: Objective To investigate the effects of rhein on the proliferation,invasion,and migration of Hep-2 cells in laryngeal carcinoma and its mechanism. Methods Laryngeal carcinoma Hep-2 cells were treated with low,medium,and high concentrations of rhein (6,12,and 24 μmol/L),and the proliferation,migration,and invasion capabilities of Hep-2 cells were detected by MTT,scratch,and Transwell assays. The expression levels of β-catenin,Dvl2,GSK-3β,and p-GSK-3β in Hep-2 cells were detected by quantitative real-time PCR and Western blotting. Results As the rhein concentration increased,the rates of proliferation,migration,and invasion of Hep-2 cells were significantly reduced (P< 0.05). Rhein downregulated the expression levels of Dvl2,p-GSK-3β,and β-catenin in Hep-2 cells (P< 0.05) and increased GSK-3β expression (P< 0.05). Activation of the Wnt/β-catenin signaling pathway by SKL2001 reversed the inhibitory effect of rhein on the migration and invasion of Hep-2 cells (P< 0.05). Inhibition of the Wnt/β-catenin signaling pathway by XAV939 inhibited the expression levels of Dvl2,p-GSK-3β,and β-catenin (P< 0.05) and further enhanced the inhibitory effect of rhein on the migration of Hep-2 cells (P< 0.05). Conclusion Rhein could effectively inhibit the proliferation,invasion,and migration of Hep-2 cells through the inhibition of the Wnt/β-catenin signaling pathway.
Key words: laryngeal carcinoma cell, rhein, proliferation, invasion, Wnt/β-catenin signaling pathway
PDF全文下载地址:

https://journal.cmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=2988