摘要： 目的 探究长链非编码RNA （lncRNA） SNHG7在前列腺癌细胞中的恶性调控机制。方法 利用癌症基因组图谱数据库对SNHG7在前列腺癌组织和癌旁组织中的表达进行比对，并分析SNHG7表达对患者生存时间的影响。采用荧光原位杂交实验检测SNHG7的亚细胞分布；利用转染技术构建SNHG7敲低细胞系，通过MTT实验和Transwell实验检测细胞的增殖和迁移能力；采用Western blotting检测ROCK1的表达，采用共转染实验检测SNHG7和ROCK1在前列腺癌细胞中的调控关系。结果 SNHG7在前列腺癌组织和细胞系中高表达（P < 0.05），并与患者生存时间缩短相关（P < 0.01）。SNHG7主要位于细胞质；敲低SNHG7后前列腺癌PC-3细胞增殖和迁移能力下降；敲低SNHG7可抑制ROCK1表达（P < 0.05）；敲低SNHG7引起的细胞增殖和迁移能力下降可被过表达ROCK1恢复。结论 敲低SNHG7可通过抑制ROCK1表达降低前列腺癌细胞的增殖和迁移能力。

敲低lncRNA SNHG7通过抑制ROCK1降低前列腺癌细胞增殖和迁移能力

佟丽斐¹, 张霞², 杨玉蝶³, 李起征³, 刘鹏⁴

1. 大连医科大学附属大连市第五人民医院放疗科, 辽宁 大连 116021;
2. 大连医科大学附属大连市第五人民医院肿瘤内科, 辽宁 大连 116021;
3. 大连医科大学附属第一医院肿瘤科, 辽宁 大连 116011;
4. 大连医科大学附属大连市中心医院肿瘤内科, 辽宁 大连 116033

收稿日期:2021-08-27出版日期:2022-04-20发布日期:2022-05-05
通讯作者:刘鹏E-mail:liupeng0823@126.com
作者简介:佟丽斐(1985-),女,主治医师,硕士研究生.
基金资助:辽宁省高等学校基本科研项目 （LQ2017024）；辽宁省自然科学基金 （201602218）；2020年大连市属医疗卫生机构医学科研项目 （2011012）


关键词: SNHG7, ROCK1, 增殖, 迁移, 前列腺癌
Abstract: Objective To explore the malignant regulation mechanism of long noncoding RNA (lncRNA) SNHG7 in prostate cancer cells. Methods The expression of SNHG7 in prostate cancer and the adjacent tissues was compared using The Cancer Genome Atlas database,and its effect on patient survival was analyzed. SNHG7 knockdown cell lines were constructed by transfection technology. A fluorescence in situ hybridization assay was used to detect its location. Cell proliferation and migration ability were detected by MTT and Transwell assays. ROCK1 expression was detected by Western blotting,while the regulatory relationship between SNHG7 and ROCK1 in prostate cancer cells was detected by a co-transfection assay. Results SNHG7 was highly expressed in prostate cancer tissues and cell lines (P < 0.05) and associated with reduced patient survival (P < 0.01). SNHG7 was mainly located in the cytoplasm. The proliferation and migration abilities of PC-3 cells decreased after SNHG7 knockdown (P < 0.01). Knocking down SNHG7 inhibited ROCK1 expression (P < 0.05). The reduction in cell proliferation and migration induced by SNHG7 knockdown was recovered by ROCK1 overexpression. Conclusion SNHG7 knockdown can reduce the proliferation and migration of prostate cancer cells by inhibiting ROCK1 expression.
Key words: SNHG7, ROCK1, proliferation, migration, prostate cancer
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