摘要： 目的 探讨长链非编码RNA SLC25A25的反义RNA1 （SLC25A25-AS1）对喉癌细胞侵袭和迁移的影响及其机制。方法 采用实时荧光定量PCR检测人鼻咽上皮细胞NP69和喉癌细胞（TU-212、TU-177和Hep-2）的SLC25A25-AS1水平，分别采用MTT法、划痕实验和Transwell实验检测细胞的增殖活性、迁移能力和侵袭能力。采用Western blotting检测信号转导及转录激活因子3 （STAT3）、磷酸化STAT3 （p-STAT3）和血管内皮生长因子（VEGF）的蛋白表达水平。结果 喉癌细胞的SLC25A25-AS1表达水平低于NP69细胞且差异有统计学意义（P < 0.05）。过表达SLC25A25-AS1使Hep-2细胞增殖活性显著降低（P < 0.05），并显著抑制Hep-2细胞的迁移和侵袭能力（P < 0.05）。过表达SLC25A25-AS1的Hep-2细胞中，p-STAT3和VEGF的蛋白表达水平显著降低（P < 0.05）。结论 SLC25A25-AS1过表达可抑制喉癌Hep-2细胞的增殖、迁移和侵袭，其抑癌机制可能与STAT3/VEGF通路有关。

长链非编码RNA SLC25A25-AS1对喉癌细胞侵袭和迁移的影响及其机制

孙倩, 刘盛力, 马钰, 杜莉

中国医科大学附属第四医院耳鼻咽喉科, 沈阳 110032

收稿日期:2021-12-06出版日期:2022-04-20发布日期:2022-05-05
通讯作者:杜莉E-mail:duli_ent@163.com
作者简介:孙倩(1989-),女,医师,硕士研究生.
基金资助:辽宁省自然科学基金 （20170541000）


关键词: 喉癌, 长链非编码RNA, SLC25A25-AS1, 信号转导及转录激活因子3, 血管内皮生长因子
Abstract: Objective To investigate the effect of antisense RNA1 of long noncoding RNA (lncRNA) SLC25A25 (SLC25A25-AS1) on the invasion and migration of laryngeal carcinoma cells and its mechanism. Methods The levels of SLC25A25-AS1 in human nasopharyngeal epithelial cells NP69 and laryngeal carcinoma cells TU-212,TU-177,and Hep-2 were detected by real-time fluorescent quantitative polymerase chain reaction. The proliferation activity,scratch healing rate,and number of membrane-penetrating cells were measured by MTT,scratch-wound,and Transwell assays,respectively. The expressions of signal transduction and activator of transcription 3 (STAT3),p-STAT3,and vascular endothelial growth factor (VEGF) proteins were detected by Western blotting. Results The level of SLC25A25-AS1 in laryngeal cancer cells was significantly lower than that in NP69 cells (P < 0.05). Overexpression of SLC25A25-AS1 significantly reduced the proliferation,migration,and invasion activity of HEP-2 cells (P < 0.05). The levels of p-STAT3 and VEGF proteins in HEP-2 cells after SLC25A25-AS1 overexpression significantly decreased (P < 0.05). Conclusion SLC25A25-AS1 overexpression can inhibit the proliferation,migration,and invasion of laryngeal cancer Hep-2 cells,and this tumor suppressive effect may be through the STAT3/VEGF pathway.
Key words: laryngeal carcinoma, long noncoding RNA, SLC25A25-AS1, signal transduction and activator of transcription 3, vascular endothelial growth factor
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