摘要： 目的 探讨脂质运载蛋白2（LCN2）在乳腺癌细胞和组织中的表达、定位，及对乳腺癌细胞侵袭转移的影响。方法 采用Western blotting检测LCN2在乳腺癌细胞系中的蛋白质表达情况，免疫组织化学检测人乳腺癌组织中LCN2的表达及定位，激光共聚焦扫描显微镜检测内源LCN2在乳腺癌细胞中的表达及定位。构建过表达LCN2的MCF-7、T47D细胞系，采用Western blotting检测与上皮间质转化（EMT）发生相关的标志物E-钙黏连蛋白（E-cadherin）、紧密连接蛋白（Claudin-1）、波形蛋白（Vimentin）、锌指转录因子（Slug）的蛋白表达情况。Transwell检测LCN2对乳腺癌细胞T47D迁移能力的影响。结果 LCN2在MCF-7，T47D及ZR-75-30乳腺癌细胞系中低表达，在MDA-MB-231中中等表达，在ZR-75-1细胞系中高表达；免疫组织化学以及激光共聚焦扫描显微镜证实LCN2主要定位于细胞质；成功构建了过表达LCN2的乳腺癌细胞系MCF-7和T47D，Western blotting检测发现在过表达LCN2的T47D细胞系中E-cadherin和Claudin-1蛋白表达明显降低，而Slug以及Vimentin表达明显增高。过表达LCN2的T47D细胞系迁移能力增强。结论 LCN2在不同乳腺癌细胞系中表达水平不同，主要定位于乳腺癌细胞质，LCN2能促进乳腺癌细胞的迁移，可能通过诱发EMT促进乳腺癌细胞的侵袭转移。

脂质运载蛋白2在乳腺癌侵袭转移中的作用机制

叶芸, 李彦姝, 张红艳, 李丰

中国医科大学生命科学学院分子细胞生物学教研室, 国家卫生健康委员会细胞生物学重点实验室暨教育部医学细胞生物学重点实验室, 沈阳 110122

收稿日期:2021-03-15出版日期:2022-02-28发布日期:2022-01-05
通讯作者:李丰E-mail:lifeng@cmu.edu.cn
作者简介:叶芸(1996-),女,硕士研究生.
基金资助:国家自然科学基金（81902701）；辽宁省自然科学基金（20180530037）


关键词: 脂质运载蛋白2, 乳腺癌, 表达, 定位, 上皮间质转化
Abstract: Objective To investigate the expression and localization of lipocalin-2 (LCN2) in breast cancer and its role in invasion and metastasis. Methods The expression and localization of LCN2 in breast cancer cell lines was detected by western blotting and immunohistochemistry and laser confocal microscopy, respectively. T47D breast cancer cells overexpressing LCN2 were cultured and detected by markers E-cadherin, claudin-1, vimentin, and slug. The effect of LCN2 on the migration of T47D breast cancer cells was detected by transwell. Results The expression of LCN2 was low in the MCF-7, T47D, and ZR-75-30 breast cancer cell lines;medium in the MDA-MB-231 breast cancer cell line;and high in the ZR-75-1 breast cancer cell line. Immunohistochemistry and laser confocal scanning microscopy demonstrated that LCN2 was localized in the cytoplasm. Western blotting analysis showed that the expression of E-cadherin and claudin-1 was significantly decreased in the T47D cells overexpressing LCN2, while the expression of slug and vimentin was significantly increased. The migration of the T47D cell line overexpressing LCN2 was promoted. Conclusion LCN2 is expressed at different levels in different breast cancer cell lines. It is mainly located in the cytoplasm and promotes migration of breast cancer cells. LCN2 expression contributes to epithelial-mesenchymal transition development in breast cancer cells.
Key words: lipocalin-2, breast cancer, expression, location, epithelial-mesenchymal transition
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