摘要： 目的 探讨阿尔茨海默病（AD）中差异长链非编码RNA （lncRNA）与靶向微RNA （miRNA）、mRNA的相互作用关系及其导致AD的发病机制。方法 在基因表达综合数据库（GEO）中下载数据，筛选出在AD早中晚期存在差异表达的lncRNA和mRNA，运用RegRNA预测lncRNA的靶向miRNA，TargetScan，TargetMiner，miRDB预测miRNA的靶向mRNA，DAVID对mRNA进行富集分析和京都基因与基因组百科全书（KEGG）通路分析，cytoscape进行网络图构建，对差异表达的mRNA进行蛋白质相互作用（PPI）分析并运用cytoscape找出关键基因。结果 从数据集GSE28146中早中晚差异表达的侯选差异基因（pDEGs）中筛选出LINC02047、LINC01124、LINC00582和LINC02478，预测差异lncRNA的下游靶miRNA有hsa-mir-132-3p、hsa-mir-1254、hsa-mir-3612、hsa-mir-4640-5p、hsa-mir-4690-3p和hsa-mir-4786-3p，然后预测靶miRNA的下游mRNA并构建AD的lncRNA-miRNA-mRNA调节网络。结论 LINC02047、LINC01124和LINC02478通过对miR-4060、miR-4090、miR-4786、miR-3612、miR-1254和miR-132的调控影响AD的发生发展。

基于生物信息学构建阿尔茨海默病内源竞争RNA调控网络

曲浩宁, 朱文韬, 何群

中国医科大学生命科学学院生物信息学教研室, 沈阳 110122

收稿日期:2021-04-22出版日期:2022-02-28发布日期:2022-01-05
通讯作者:何群E-mail:qunh@cmu.edu.cn
作者简介:曲浩宁(2000-),男,本科在读.
基金资助:辽宁省大学生创新创业基金（S202010159033）


关键词: 阿尔茨海默病, 内源竞争RNA, 长链非编码RNA, 生物信息学
Abstract: Objective To explore the interaction between differential long noncoding RNA (lncRNA) and targeted microRNA (miRNA) and messenger RNA (mRNA) in Alzheimer's disease (AD) and AD pathogenesis. Methods The data were downloaded from the GEO database. Differentially expressed lncRNA and mRNA in the early, middle, and late stages of AD were screened. The targeted miRNA of lncRNA was predicted using the RegRNA web server. The targeted mRNA of miRNA was predicted using TargetScan, TargetMiner, and miRDB. DAVID was used for mRNA enrichment and KEGG pathway analyses. The resulting network was constructed using Cytoscape. Protein-protein interaction analysis of differentially expressed mRNA was performed. The key genes were identified using Cytoscape. Results Select LINC02047, LINC01124, LINC00582, LINC02478 from the pDEFs differentially expressed in the early, mid-late and late stages in the data set GSE28146. Predict the downstream target miRNA of the differential lncRNA, and then predict the downstream mRNA of the target miRNA and construct the lncRNA-miRNA-mRNA regulatory network of AD. Conclusion LINC02047, LINC01124, LINC02478 may influence the occurrence and development of AD through the regulation of miR-4060, miR-4090, miR-4786, miR-3612, miR-1254, and miR-132.
Key words: Alzheimer's disease, competing endogenous RNAs, long non-coding RNA, bioinformatics
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