摘要： 目的 利用M1型巨噬细胞极化模型，探讨全反式维甲酸（ATRA）对M1型巨噬细胞极化的影响。方法 选用8周龄C57BL/6雌性小鼠，提取并纯化腹腔巨噬细胞，随机分为M组、M1组、DMSO组和ATRA组。脂多糖（LPS，100 ng/mL）联合干扰素-γ（IFN-γ，20 ng/mL）诱导巨噬细胞构建M1型巨噬细胞极化模型，ATRA（30 μg/mL）处理24 h。Annexin V-PE/7-AAD双染法检测ATRA对巨噬细胞凋亡的影响，流式细胞仪检测F4/80⁺iNOS⁺M1型细胞的表达，实时PCR检测白细胞介素-1β（IL-1β）、诱导型一氧化氮合酶（iNOS）、肿瘤坏死因子-α（TNF-α）的mRNA表达。结果 与M1组相比，ATRA组中巨噬细胞凋亡率无明显变化（P>0.05），F4/80⁺iNOS⁺M1型细胞比例明显降低（P<0.05）；IL-1β、iNOS、TNF-α mRNA表达明显降低（P<0.05）。结论 ATRA对巨噬细胞无细胞毒作用且可抑制巨噬细胞向M1型的极化。

全反式维甲酸对M1型巨噬细胞极化的影响

薛高峰¹, 王琳源¹, 关宁², 高秀秋¹

1. 锦州医科大学附属第二医院牙周黏膜科, 辽宁 锦州 121000;
2. 锦州医科大学附属第一医院脑与脊髓损伤重点实验室, 辽宁 锦州 121000

收稿日期:2021-03-11出版日期:2022-01-30发布日期:2021-12-30
通讯作者:王琳源E-mail:wanglinyuan2006@163.com
作者简介:薛高峰(1996-),女,硕士研究生.
基金资助:国家自然科学基金青年基金（81600870）；辽宁省自然科学基金（2019-ZD-0824，2021-MS-324）


关键词: 全反式维甲酸, M1型巨噬细胞, 巨噬细胞极化
Abstract: Objective To use the polarization model of M1 macrophages to investigate the effect of all-trans retinoic acid (ATRA) on the polarization of M1 macrophages. Methods Eight-week-old C57BL/6 female mice were used to extract and purify peritoneal macrophages, and they were randomly divided into the M, M1, DMSO, and ATRA groups. Lipopolysaccharide (LPS, 100 ng/mL) combined with interferon-γ (IFN-γ, 20 ng/mL) was used to induce macrophages to construct an M1 type macrophage polarization model that was subjected to ATRA (30 μg/mL) treatment for 24 h. The Annexin V-PE/7-AAD double staining method was used to detect the effect of ATRA on macrophage apoptosis, flow cytometry used to detect the expression of F4/80⁺iNOS⁺M1 type cells, and real-time PCR used to detect interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), and tumor necrosis factor-α (TNF-α) mRNA expression. Results Compared with the M1 group, there was no significant change in the apoptosis rate of macrophages in the ATRA group (P>0.05), and the proportion of F4/80⁺iNOS⁺M1 type cells was significantly reduced (P<0.05). The mRNA expression of IL-1β, iNOS, and TNF-α was significantly reduced (P<0.05). Conclusion ATRA has no cytotoxic effect on macrophages and can inhibit their polarization to the M1 type.
Key words: all-trans retinoic acid, M1 type macrophages, macrophage polarization
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