摘要： 目的 探讨317L-Cu抗菌不锈钢（317L-Cu SS）对小鼠成纤维细胞（L-929细胞）增殖、黏附、凋亡等生物学行为的影响，评价抗菌不锈钢材料的细胞毒性；同时观察不同浓度Cu²⁺对细胞毒性的影响。方法 检测L-929细胞在普通医用不锈钢（317L SS）、317L-Cu SS、医用纯钛（Ti）及纯铜（Cu）材料浸提液中培养24 h、48 h、72 h时的光密度（OD）值，判断细胞毒性级别；应用流式细胞仪Annexin V-FITC/PI双染法检测细胞凋亡情况；吖啶橙染色法检测L929细胞在材料表面的黏附情况。结果 317L-Cu SS组24 h、72 h OD值、细胞黏附数量均低于Ti组而高于317L SS组（均P<0.05）。317L-Cu SS组细胞毒性分级为1级，Cu组细胞毒性分级为3级或4级；与317L SS组比较，317L-Cu SS组细胞凋亡率显著降低（P<0.05）。而与阴性对照组、Ti组比较，317L-Cu SS组细胞凋亡率无统计学差异（均P>0.05）。纯铜组细胞凋亡率明显高于其他各组（均P<0.05）。结论 317L-Cu抗菌不锈钢对细胞的增殖、凋亡、黏附等生物学行为无明显不良影响，生物相容性良好，而纯铜材料细胞毒性明显，Cu²⁺的细胞毒性呈双向性。

317L-Cu抗菌不锈钢对小鼠成纤维细胞生物学行为的影响

冯靖雯¹, 杨泽¹, 杨柯², 张扬¹

1. 中国医科大学口腔医学院·附属口腔医院正畸科, 辽宁省口腔疾病重点实验室, 沈阳 110002;
2. 中国科学院金属研究所前沿材料研究部, 沈阳 110016

收稿日期:2021-05-12出版日期:2022-01-30发布日期:2021-12-30
通讯作者:张扬E-mail:kp3666@126.com
作者简介:冯靖雯(1987-),女,主治医师,硕士.



关键词: 含铜不锈钢, 生物材料, 小鼠, 成纤维细胞, 生物学行为
Abstract: Objective To observe the effects of 317L-Cu antibacterial stainless steel (317L-Cu SS) on the biological behaviors of mouse fibroblasts (L-929 cells), including proliferation, adhesion, and apoptosis;evaluate the cytotoxicity of antibacterial stainless steel;and observe the effects of different concentrations of Cu²⁺ on cytotoxicity. Methods The optical density (OD) values of the L-929 cells were measured at 24 h, 48 h, and 72 h in ordinary medical-grade stainless steel (317L SS), 317L-Cu SS, medical pure titanium (Ti), and pure copper (Cu) material extracts to determine the cytotoxicity level. The flow cytometry Annexin V-FITC/PI double staining method was applied to detect apoptosis. Acridine orange staining was applied to detect the adhesion of L929 cells on the material. Results The 24 h and 72 h OD values and cell adhesion numbers were lower in the 317L-Cu SS group than in the Ti group (all P<0.05) and higher than those of the 317L SS group (P<0.05). The 317L-Cu SS group had a cytotoxicity grade of 1, while the Cu group had cytotoxicity grades of 3 or 4. Apoptosis rate was significantly lower in the 317L-Cu SS group than in the 317L SS group (P<0.05). There was no significant difference in the apoptosis rate of the 317L-Cu SS group versus the negative control and Ti groups (all P>0.05). The apoptosis rate was significantly higher in the Cu group than in the other groups (P<0.05). Conclusion The 317L-Cu SS material was biocompatible and had no significant adverse effects on biological behaviors such as cell proliferation, apoptosis, and adhesion, whereas the cytotoxicity of the Cu material was significant and that of Cu²⁺ was bidirectional.
Key words: copper-containing stainless steel, biomaterials, mice, fibroblasts, biological behavior
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