摘要： 目的 探讨人羊膜提取物对小鼠胰岛缺氧损伤的保护作用。方法 分离小鼠原代胰岛，随机分为缺氧培养组、缺氧+人羊膜提取物处理组、正常氧气培养组及假手术组，培养12 h后检测胰岛活性和葡萄糖刺激胰岛素分泌功能，探讨人羊膜提取物保护胰岛的分子机制。体外培养胰岛进行同基因小鼠移植，观察糖尿病受体小鼠血糖、体质量、腹腔注射糖耐量和胰岛移植物病理。结果 缺氧明显降低了胰岛活性和胰岛素分泌功能，人羊膜提取物能显著改善上述功能，差异有统计学意义（P < 0.05）。人羊膜提取物显著提高缺氧胰岛细胞内核因子相关因子2（Nrf2）与血红素加氧酶1（HO-1）表达水平（P < 0.05）。经人羊膜提取物预处理的缺氧胰岛移植后能明显降低糖尿病受体小鼠血糖，增加体质量，改善腹腔注射糖耐量，差异有统计学意义（P < 0.05）。结论 人羊膜提取物对缺氧胰岛的损伤有明显保护作用，并能够改善胰岛移植物的功能。

人羊膜提取物对小鼠胰岛缺氧损伤的保护作用及其分子机制

郭庭维, 杨召铭, 李峰, 林建贞, 张城硕, 孙宁, 李晓航, 张佳林

中国医科大学附属第一医院肝胆外科, 沈阳 110001

收稿日期:2021-04-12出版日期:2021-12-30发布日期:2021-12-09
通讯作者:张佳林E-mail:jlz2000@yeah.net
作者简介:郭庭维(1993-),男,医师,硕士.



关键词: 人羊膜提取物, 胰岛移植, 缺氧, 核因子相关因子2, 血红素加氧酶1, 1型糖尿病
Abstract: Objective To explore the protective effect of human amniotic membrane extract （hAME） against islet hypoxic injury Methods Primary mouse pancreatic islets were isolated and divided into hypoxia，hypoxia+hAME，normoxia，and naive groups. After 12-hour cultivation periods，the viability and glucose-stimulated insulin secretion function of the isolated islets were assessed to investigate the protective effects of hAME. The potential mechanism of islet protection was explored by Western blotting. Moreover，cultured islets were transplanted in the recipient mice，then blood glucose and body weight were detected，and intraperitoneal glucose tolerance graft histopathological tests were performed. Results Hypoxia significantly reduced the viability and insulin secretion function of isolated islets，while supplementation of hAME ameliorated this effect （P < 0.05）. hAME significantly increased nuclear erythroid 2-related factor 2 （Nrf2） and heme oxygenase-1 （HO-1） expression in hypoxia-stressed islet cells （P < 0.05）. Moreover，diabetic recipient mice transplanted with hAME-treated hypoxia islets had significantly lower blood glucose levels，higher body weight，and better metabolic function of islet grafts compared to those transplanted with hypoxia-stressed islets. Conclusion hAME has obvious protective effect on islet damage caused by hypoxia，and improves islet graft functions.
Key words: human amniotic membrane extract, islet transplantation, hypoxia, nuclear erythroid 2-related factor 2, heme oxygenase-1, type 1 diabetes mellitus
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