摘要： 目的 探讨WWP2基因对急性淋巴细胞白血病（ALL）细胞系增殖与凋亡的影响。方法 通过转染分别在ALL细胞系Jurkat中敲减和过表达WWP2基因。在多柔比星（dox）作用下，通过细胞增殖试验、流式细胞术及ROS染色等实验方法检测Jurkat细胞增殖、凋亡情况。结果 敲减组细胞增殖活性降低，细胞凋亡率增高，产生活性氧物质增多；过表达组细胞增殖活性升高，细胞凋亡率降低，产生活性氧物质减少。敲减了WWP2基因的Jurkat细胞对dox更敏感，而过表达WWP2基因的Jurkat细胞对dox敏感性降低。结论 WWP2能够增强ALL细胞系增殖能力，减少细胞凋亡，可能作为癌基因调控ALL的发生发展。WWP2基因的表达水平能影响Jurkat细胞对dox药物的敏感性，对临床治疗有一定指导作用。

WWP2在急性淋巴细胞白血病细胞系中的表达及其对多柔比星药物敏感性的影响

徐海琪, 黄心悦, 张丽君

中国医科大学附属第一医院血液内科, 沈阳 110001

收稿日期:2021-01-06出版日期:2021-10-30发布日期:2021-10-11
通讯作者:张丽君E-mail:lzhang202003@163.com
作者简介:徐海琪(1994-),女,硕士研究生.



关键词: WWP2, 急性淋巴细胞白血病, 完全缓解, 凋亡, 多柔比星
Abstract: Objective To observe the effect of WWP2 gene on proliferation and apoptosis of acute lymphoblastic leukemia (ALL) cell line Jurkat after knocked down and overexpressed WWP2 gene. Methods With the stimulating of chemotherapeutic drug doxorubicin (dox), we tested the proliferation and apoptosis of the transfected Jurkat cells by CCK-8, flow cytometry and ROS staining. Results In the group of cells knocked down WWP2 gene, the cells proliferation activity decreased, the apoptosis rate increased and the reactive oxygen content decreased; in the group of cells overexpressed WWP2 gene, the cells proliferation activity increased, the apoptosis rate decreased and the reactive oxygen content increased. Interestingly, we also infer that WWP2 gene may partially regulate the damage caused by dox to ALL cells. Cells with low WWP2 expression were more sensitive to dox, on the contrary, cells with high WWP2 expression were less sensitive to dox. Conclusion WWP2 may develop and regulate ALL as an oncogene, it can enhance the proliferation and reduce the apoptosis of ALL cells line. Furthermore, the level of WWP2 gene expression in ALL patients may suggest some treatment.
Key words: WWP2, acute lymphoblastic leukemia, complete remission, apoptosis, doxorubicin
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