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褪黑素对爆震伤诱导小鼠肺损伤的保护作用及机制

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摘要: 目的 探讨褪黑素对爆震伤诱导小鼠肺损伤的保护作用及机制。方法 将30只C57BL/6小鼠随机分为假手术(Sham)组、爆震伤(Blast)组及爆震伤+褪黑素(Blast+Mel)组。爆震伤后48 h收集小鼠肺组织标本。采用苏木精-伊红(HE)染色、酶联免疫吸附实验(ELISA)和蛋白质印迹实验(Western blotting)检测褪黑素对肺爆震伤的保护作用。结果 与Blast组小鼠相比,褪黑素能够显著降低小鼠肺湿重/干重比和血清白细胞介素-4(IL-4)及肿瘤坏死因子-α(TNF-α)的表达水平;改善小鼠肺组织病理学变化;降低肺组织白细胞介素-6(IL-6)、白细胞介素-17(IL-17)和白细胞介素-23(IL-23)表达水平。同时,褪黑素明显抑制爆震伤导致的小鼠重组人Semaphorin 7a蛋白(Sema7a)和人胎球蛋白A(Ahsg)蛋白表达;显著降低小鼠肺组织P38蛋白(p38)和核因子κB(NF-κB)通路蛋白表达。结论 褪黑素可能通过抑制p38/NF-κB信号通路发挥抗炎活性,进而对爆震伤诱导的肺损伤进行保护。

褪黑素对爆震伤诱导小鼠肺损伤的保护作用及机制

施琳, 赵剑
沈阳药科大学生命科学与生物制药学院药理教研室, 沈阳 110016
收稿日期:2021-01-26出版日期:2021-09-30发布日期:2021-09-18
通讯作者:赵剑E-mail:zll0625@sohu.com
作者简介:施琳(1986-),女,主管技师,本科.
基金资助:辽宁省自然科学基金(20170540947)


关键词: 爆震伤, 褪黑素, 肺损伤, 炎症
Abstract: Objective To investigate the protective effect and mechanism of melatonin against blast-induced lung injury. Methods Thirty C57BL/6 mice were randomly divided into three groups:sham group,blast group,and blast + melatonin group. The lung tissue samples of mice were collected 48 hours after blast injury. The protective effect of melatonin on lung blast injury was detected by hematoxylin and eosin (HE) staining,enzyme-linked immunosorbent assay,and western blotting. Results Compared with the blast injury group,melatonin significantly reduced the lung wet weight/dry weight ratio and serum IL-4 and TNF-α expression,improved the pathological changes of lung tissue,and decreased the expression of interleukin (IL)-6,IL-17,IL-23 in lung tissues of the blast + melatonin group. Meanwhile,melatonin significantly inhibited Sema7a and Ahsg protein expression and reduced the levels of p38 and NF-κB protein. Conclusion Melatonin may exert anti-inflammatory effects by inhibiting the p38/NF-κB signaling pathway,protecting against lung injury induced by blast injury.
Key words: blast injury, melatonin, lung injury, inflammation
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https://journal.cmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=2836
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