褪黑素对爆震伤诱导小鼠肺损伤的保护作用及机制
施琳, 赵剑沈阳药科大学生命科学与生物制药学院药理教研室, 沈阳 110016
收稿日期:
2021-01-26出版日期:
2021-09-30发布日期:
2021-09-18通讯作者:
赵剑E-mail:zll0625@sohu.com作者简介:
施琳(1986-),女,主管技师,本科.基金资助:
辽宁省自然科学基金(20170540947)关键词: 爆震伤, 褪黑素, 肺损伤, 炎症
Abstract: Objective To investigate the protective effect and mechanism of melatonin against blast-induced lung injury. Methods Thirty C57BL/6 mice were randomly divided into three groups:sham group,blast group,and blast + melatonin group. The lung tissue samples of mice were collected 48 hours after blast injury. The protective effect of melatonin on lung blast injury was detected by hematoxylin and eosin (HE) staining,enzyme-linked immunosorbent assay,and western blotting. Results Compared with the blast injury group,melatonin significantly reduced the lung wet weight/dry weight ratio and serum IL-4 and TNF-α expression,improved the pathological changes of lung tissue,and decreased the expression of interleukin (IL)-6,IL-17,IL-23 in lung tissues of the blast + melatonin group. Meanwhile,melatonin significantly inhibited Sema7a and Ahsg protein expression and reduced the levels of p38 and NF-κB protein. Conclusion Melatonin may exert anti-inflammatory effects by inhibiting the p38/NF-κB signaling pathway,protecting against lung injury induced by blast injury.
Key words: blast injury, melatonin, lung injury, inflammation
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