摘要： 目的 探讨三七治疗缺血性脑卒中的有效成分、靶点及通路，以及可能的分子机制。方法 通过中药系统药理学分析平台获取三七活性成分；利用UniProtKB数据库、DisGeNET数据库筛选出三七治疗缺血性脑卒中关联的作用靶标；采用Cytoscape 3.6.0软件建立三七有效成分-缺血性脑卒中作用靶标网络；通过DAVID数据库构建作用靶标之间互相作用网络，获得京都基因与基因组百科全书（KEGG）通路、基因肿瘤学（GO）分类富集分析结果，建立有效成分-作用靶标-信号通路网络。结果 筛选出了三七治疗缺血性脑卒中5个有效成分（豆甾醇、β-谷固醇、人参皂苷rh2、槲皮素、三七皂苷r1），141种交集靶标，5个核心靶标（白细胞介素-6、蛋白激酶B、c-JUN蛋白、肝磷脂结合表皮生长因子、血管内皮生长因子A）。低氧诱导因子-1通路、磷酯酰肌醇3激酶/蛋白激酶B通路、丝裂原活化蛋白激酶通路是三七治疗缺血性脑卒中的重要信号通路。结论 三七通过自身有效成分作用多个靶标基因治疗缺血性脑卒中，其作用机制可能是通过信号通路获得非常复杂的网络药理调节完成的。

基于网络药理学三七治疗缺血性脑卒中的作用机制研究

伍海军¹, 吴静澜², 杨欣², 杨华¹, 潘政¹, 陈永顺¹

1. 贵州中医药大学第一附院医院神经外科, 贵阳 550001;
2. 贵州中医药大学药学院中药学教研室, 贵阳 550025

收稿日期:2020-11-30出版日期:2021-09-30发布日期:2021-09-18
通讯作者:杨欣E-mail:5591609@qq.com
作者简介:伍海军(1981-),男,副主任医师,硕士.
基金资助:贵州省科技合作计划（黔科合基础[2019]1028）


关键词: 三七, 缺血性脑卒中, 网络药理学, 作用机制
Abstract: Objective To study the effective components,targets,and pathways of Sanqi in the treatment of ischemic stroke,and to explore its possible molecular mechanism. Methods The active ingredients of Sanqi were obtained by using a network pharmacology and Chinese medicine systematic pharmacology analysis platform. UniProtKB database,DisGeNET database,and Excel 2010 software were used to screen out the associated targets of Sanqi in the treatment of ischemic stroke. Cytoscape 3.6.0 software was used to establish the active component-ischemic stroke target network of Sanqi. We used the Database for Annotation,Visualization and Integrated Discovery (DAVID) to build a target interaction between the networks.We also performed Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis and gene ontology (GO) classification. We investigated the role of notoginseng in the treatment of ischemic cerebral apoplexy,mainly the signaling pathways and the related molecular functions,and at the same time,established an "active ingredient-target-signaling pathway" network. Results Five effective components (stigmasterol,beta-sitosterol,ginsenoside rh2,quercetin,and notoginsenoside r1) of Sanqi acting on ischemic stroke and 41 intersection targets were obtained by the above method. There were five core targets:interleukin-6 (IL-6),protein kinase B (AKT),C-Jun protein (C-Jun),heparin-binding epidermal growth factor (HB-EGF),and vascular endothelial growth factor A (VEGFA),and these are mainly used to treat ischemic stroke by regulating and controlling signaling pathways and changing protein binding. Conclusion Sanqi,an important drug in the treatment of ischemic stroke,can have multiple effects by acting on multiple targets,and can also obtain very complex pharmacological regulatory effects through the signaling pathway.
Key words: Sanqi, cerebral ischemic stroke, network pharmacology, mechanism of action
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