摘要： 目的 分析子痫前期患者胎盘组织中环状RNA（circRNA）的表达，为子痫前期分子机制的进一步研究提供生物信息学依据。方法 从GEO数据库下载子痫前期患者和正常对照孕妇胎盘组织的circRNA芯片数据集，通过生物信息学分析，筛选差异表达基因（DEGs），联合分析差异基因，并预测潜在结合的微RNA（miRNA）。结果 联合分析筛选出10个DEGs，均为子痫前期患者胎盘组织下调基因，主要定位在人类第7号染色体（chr7），其中7q36及7q22.1与子痫前期的发生和发展关系密切。在circRNA作为竞争性内源RNA（ceRNA）潜在结合的miRNA中，预测miR-1207-3p与疾病关系密切，并可调控细胞增殖、迁移、凋亡等功能。结论 circRNA在子痫前期的发生、发展及预测中有重要作用，目前的生物信息学分析为探索进一步的疾病机制提供了研究基础。

子痫前期患者胎盘组织中环状RNA差异表达的生物信息学分析

佟春晓, 陈海英, 孙曼妮, 孟涛

中国医科大学附属第一医院产科, 沈阳 110001

收稿日期:2021-05-11出版日期:2021-08-30发布日期:2021-07-29
通讯作者:孟涛E-mail:cmumt@163.com
作者简介:佟春晓(1991-),女,医师,硕士.
基金资助:国家自然科学基金（81871173）


关键词: 子痫前期, 生物信息学分析, 环状RNA
Abstract: Objective To analyze the expression of circular RNAs (circRNAs) in the placentas of patients with pre-eclampsia (PE) and provide a bioinformatic basis for further studies of the molecular mechanisms underlying PE. Methods The microarray datasets of different preeclamptic placental tissues of PE patients and normal pregnant women were downloaded from the GEO database. Differentially expressed genes (DEGs) were screened and analyzed by bioinformatics analysis jointly,and the potential microRNAs (miRNAs) were predicted. Results Ten DEGs were screened by combined analysis,all of which were downregulated in the placenta from PE patients and mainly distributed on the human chromosome 7 (chr7)； among these sites on chr7,7q36,and 7q22.1 were closely related to the occurrence and development of PE. As a potential miRNA of competing endogenous RNAs (ceRNAs),miR-1207-3p is closely related to diseases and regulates the proliferation,migration,apoptosis,and other functions of cells. Conclusion circRNAs play important roles in the occurrence,development,and prediction of PE. The current bioinformatics analysis provides a research basis for the further exploration of the mechanisms underlying PE.
Key words: pre-eclampsia, bioinformatics analysis, circular RNA
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