摘要： 目的 探讨线粒体受体转位蛋白（TSPO）靶向光动力学治疗（PDT）对结肠癌的作用。方法 采用免疫组织化学及Western blotting方法检测氧化偶氮甲烷/葡聚糖硫酸钠（AOM/DSS）诱导的小鼠结肠癌组织及14个结肠癌细胞系中TSPO的表达，使用TSPO靶向光敏剂IR700DX-6T对14个结肠癌细胞系进行PDT。建立小鼠皮下移植瘤模型，进行PDT。结果 免疫组织化学结果显示，小鼠结肠癌组织中TSPO的表达明显高于癌旁正常黏膜组织。Western blotting结果证实14个结肠癌细胞系、AOM/DSS诱导的小鼠结肠癌组织及皮下移植瘤组织中的TSPO表达水平明显高于正常结肠黏膜上皮组织。以TSPO为靶向的PDT（TSPO-PDT）可诱导结肠癌细胞死亡，并呈现明显的时间及浓度依赖性（P < 0.01）。动物实验证实TSPO-PDT后原发肿瘤和远端肿瘤的生长速度明显小于对照组（P < 0.01）。结论 TSPO在结肠癌细胞系和癌组织中均呈高表达，TSPO-PDT可有效抑制结肠癌细胞生长。

线粒体受体转位蛋白靶向光动力学治疗对结肠癌的作用

肖日姣, 廖照辉, 韩赫, 魏房, 李震

中国医科大学附属第四医院第四普通外科, 沈阳 110032

收稿日期:2021-04-28出版日期:2021-08-30发布日期:2021-07-29
通讯作者:李震E-mail:cmulizhen@126.com
作者简介:肖日姣(1988-),女,护师,本科.
基金资助:辽宁省教育厅科学研究项目（JCJR2020015）


关键词: 结肠癌, 线粒体受体转位蛋白, 光动力学治疗
Abstract: Objective To investigate the effect of translocation protein (TSPO)-targeted photodynamic therapy (PDT) targeting the 18 kDA mitochondrial receptor on colorectal cancer cells. Methods The expression of TSPO in azoxymethane/dextran sodium sulfate (AOM/DSS)-induced mouse colorectal cancer (CRC) tissues and 14 CRC cell lines was detected by immunohistochemistry and western blotting. The 14 CRC cell lines were treated with IR700DX-6T,a TSPO-targeting photosensitizer. A mouse model of a subcutaneous xenograft was established for PDT. Results Immunohistochemistry confirmed that TSPO expression in mouse CRC tissues was significantly higher than that in normal adjacent mucosal tissues. Western blotting analysis confirmed that TSPO expression levels in 14 colorectal cancer cell lines,AOM/DSS-induced mouse CRC tissues,and subcutaneously transplanted tumor tissues were significantly higher than those in normal colonic mucosal epithelial tissues. TSPO-PDT induced CRC cell death and showed significant time and concentration dependence (P < 0.01). Animal experiments confirmed that the growth rate of primary and distal tumors after TSPO-PDT treatment was significantly lower than that in the control group (P < 0.01). Conclusion TSPO is highly expressed in CRC cells and colon cancer tissues. TSPO-PDT can effectively inhibit the growth of CRC.
Key words: colorectal cancer, mitochondrial receptor 18 kDA translocation protein, photodynamic therapy
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