摘要： 目的 探讨骆驼奶对噁唑酮诱导的小鼠肠炎模型中肠道黏膜免疫功能的调节作用。方法 将40只BALB/c雄性小鼠以3%噁唑酮乙醇溶液涂搽皮肤致敏后用1%噁唑酮乙醇溶液灌肠，建立小鼠肠炎模型，分为实验组和对照组，每组20只，每天分别用骆驼奶和双蒸水灌胃。记录小鼠体质量、疾病活动指数；采用流式细胞术、ELISA、实时荧光定量PCR测定肠免疫细胞功能的改变。结果 实验组与对照组相比，小鼠体质量下降减慢，疾病活动指数降低，肠道淋巴细胞中CD4⁺CD25⁺T细胞和CD4⁺IFN-γ⁺T细胞比例升高，CD4⁺IL-17⁺T细胞比例下降，细胞培养上清中IL-17、IL-4、IL-13浓度降低，IFN-γ浓度升高。结论 骆驼奶可能通过调节T细胞增殖分化和细胞因子分泌缓解噁唑酮诱导的小鼠肠炎。

骆驼奶对噁唑酮诱导的小鼠肠炎的作用

崔畅婉, 孙峥嵘

中国医科大学附属盛京医院生物样本库, 沈阳 110004

收稿日期:2020-08-31出版日期:2021-07-30发布日期:2021-06-24
通讯作者:孙峥嵘E-mail:sunzr@sj-hospital.org
作者简介:崔畅婉(1991-),女,技师,硕士.
基金资助:国家自然科学基金（81171581）


关键词: 骆驼奶, 肠炎, 噁唑酮
Abstract: Objective To investigate the regulatory effect of camel milk on intestinal mucosal immune function in oxazolone-induced colitis in mice. Methods The colitis model was constructed using 3% oxazolone ethanol solution for skin sensitization and 1% oxazolone ethanol solution by enema in male BALB/c mice. The mice were fed daily with camel milk in the experimental group (n=20) and with double steamed water in the control group (n=20). The body weight and disease activity index in mice were recorded. Flow cytometry,enzyme-linked immune sorbent assay,and real-time fluorescence quantitative PCR were used to test changes in colon immune cell function. Results Compared with the control group,the mice in the experimental group showed a slow weight loss and decreased disease activity index. Furthermore,an increased proportion of the lymphocytes CD4⁺ CD25⁺ T cells and CD4⁺ IFN-γ⁺ T cells in the colon mucosa lamina propria was exhibited in the experimental group,whereas the proportion of CD4⁺ IL-17⁺ T cells decreased. The concentration of interleukin (IL)-17,IL-4,and IL-13 in the cell culture supernatant decreased,whereas that of IFN-γ increased in the experimental group. Conclusion Camel milk may alleviate oxazolone-induced colitis in mice by regulating T cell proliferation and differentiation and cytokine secretion.
Key words: camel milk, colitis, oxazolone
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