摘要： 目的 探讨白细胞介素-9 （IL-9）及其受体（IL-9R）在非小细胞肺癌组织中的表达及其临床意义。方法 收集2017年1月至2018年12月中国医科大学附属盛京医院胸外科110例手术切除并经病理确诊的非小细胞肺癌癌组织及其癌旁正常石蜡包埋组织标本，并收集其中19例非小细胞肺癌的癌组织及其周围正常的新鲜组织标本。收集患者性别、年龄、肿瘤分化程度、肿瘤大小、淋巴结转移及TNM分期等临床资料。采用免疫组织化学法检测肺癌组织及其癌旁组织中IL-9、IL-9R的表达情况，采用Western blotting检测肺癌组织及其癌旁组织中IL-9蛋白的表达。采用χ²检验分析IL-9及IL-9R表达与肿瘤临床病理的相关性。采用t检验比较非小细胞肺癌癌组织及癌旁组织IL-9表达的差异。结果 IL-9和IL-9R在肺癌组织中的表达明显高于癌旁正常组织（P<0.05）。肺癌组织中IL-9蛋白表达水平显著高于癌旁正常组织（P<0.05）。IL-9表达水平与患者肿瘤大小、淋巴结转移及TNM分期相关（均P<0.05），IL-9R与患者TNM分期和淋巴结转移相关（均P<0.05）。结论 IL-9及IL-9R在非小细胞肺癌癌组织高表达，其表达水平与肿瘤大小、淋巴结转移以及TNM分期相关，IL-9/IL-9R可能成为非小细胞肺癌免疫治疗的新靶点。

白细胞介素-9及其受体在非小细胞肺癌组织中的表达及临床意义

高媛¹, 周妍¹, 周新佳², 郑锐¹

1. 中国医科大学附属盛京医院呼吸与重症监护内科, 沈阳 110004;
2. 中国医科大学附属盛京医院耳鼻咽喉科, 沈阳 110004

收稿日期:2020-07-24出版日期:2021-06-30发布日期:2021-05-28
通讯作者:郑锐E-mail:zhengr@sj-hospital.org
作者简介:高媛(1981-),女,主治医师,博士.
基金资助:辽宁省科技厅科学技术计划（2018225006）


关键词: 白细胞介素-9, 白细胞介素-9受体, 非小细胞肺癌
Abstract: Objective To explore the expression and clinical significance of interleukin-9 (IL-9) and interleukin-9 receptor (IL-9R) in non-small cell lung cancer tissues. Methods Between January 2017 and December 2018,paraffin-embedded specimens were prepared from cancerous and adjacent normal tissues from 110 cases of pathologically diagnosed non-small cell lung cancer,and fresh specimens of cancerous and surrounding normal tissue were collected from 19 non-small cell lung cancer patients,by surgical resection in the Department of Thoracic Surgery,Shengjing Hospital of China Medical University. Clinical data including gender,age,tumor differentiation degree,tumor size,lymph node metastasis,and TNM staging was collected for all patients. Immunohistochemistry was used to detect IL-9 and IL-9R expression in lung cancer tissues and adjacent tissues. Western blotting was used to detect IL-9 protein expression in lung cancer tissues and adjacent tissues. Correlations between IL-9 and IL-9R expression and tumor clinicopathology were assessed by χ² analysis. Student's t test was used to compare IL-9 expression in non-small cell lung cancer tissues and adjacent tissues. Results By immunohistochemistry,expression of IL-9 and IL-9R were seen to be significantly higher in lung cancer tissues than in normal tissues adjacent to cancers (P<0.05). Western blotting results showed that IL-9 protein expression was significantly higher in lung cancer tissues than in normal tissues adjacent to cancers (P<0.05). IL-9 expression levels correlated with tumor size,lymph node metastasis,and TNM stage (all P<0.05),and IL-9R expression correlated with the patient's TNM stage and lymph node metastasis (all P<0.05). Conclusion IL-9 and IL-9R are highly expressed in non-small cell lung cancer tissues,and their expression levels correlate with tumor size,lymph node metastasis,and TNM stage. IL-9/IL-9R may represent a novel target for diagnosis and treatment of non-small cell lung cancer.
Key words: interleukin-9, interleukin-9 receptor, non-small cell lung cancer
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