摘要： 目的 研究circZEB1对胃癌细胞增殖和凋亡的影响及其调控机制。方法 利用细胞核质分离实验检测circZEB1在细胞中的定位，实时PCR检测circZEB1在胃癌细胞系HGC-27、SGC-7901、AGS、MGC-823、MKN-45和人胃黏膜上皮细胞系GES1中的表达情况，CCK-8实验检测circZEB1对胃癌细胞增殖能力的影响，流式细胞术检测circZEB1对胃癌细胞凋亡和周期分布的作用，荧光素酶告基因实验验证circZEB1与hsa-miR-656的直接靶向关系。结果 circZEB1主要表达在胃癌细胞胞质中，并在多种胃癌细胞系中高表达。敲除circZEB1后显著抑制胃癌细胞增殖，并促进胃癌细胞凋亡，诱导细胞周期阻滞于G₀/G₁期，荧光素酶报告基因实验证实circZEB1能够直接靶向hsa-miR-656。结论 circZEB1通过竞争性结合hsa-miR-656，调控胃癌细胞的增殖和凋亡。

circZEB1通过竞争性结合hsa-miR-656调控胃癌细胞的增殖和凋亡

刘会缔, 李彦科, 苗鹏, 邢承忠

中国医科大学附属第一医院肛肠外科, 沈阳 110001

收稿日期:2020-11-11出版日期:2021-06-30发布日期:2021-05-28
通讯作者:邢承忠E-mail:XCZ1966@126.com
作者简介:刘会缔(1988-),男,医师,硕士.
基金资助:辽宁省自然科学基金（2019-ZD-0748）


关键词: 胃癌, 环状RNA, 增殖, 凋亡, 微RNA
Abstract: Objective To study the effect of circZEB1 on the proliferation and apoptosis of gastric cancer cells and decipher its underlying regulatory mechanism. Methods Nucleoplasmic separation experiments were used to detect the localization of circZEB1 in gastric cancer cells. Real-time PCR was used to detect the expression of circZEB1 in five gastric cancer cell lines and a physiologically epithelial cell line. CCK-8 assays and flow cytometry were used to detect the effect of circZEB1 on the proliferation,and the apoptosis and cycle distribution of gastric cancer cells,respectively. Fluorescein plum reporter assays were employed to verify the direct physical interaction between circZEB1 and hsa-miR-656. Results circZEB1 is mainly expressed in the cytoplasm of gastric cancer cells in addition to being highly expressed in gastric cancer cell lines. Knockout of circZEB1 in gastric cancer cells significantly inhibits the proliferation,promotes the apoptosis,and induces cell cycle arrest at G₀/G₁ phase. The fluorescein plum reporter assays confirmed that circZEB1 directly targets hsa-miR-656. Conclusion circZEB1 regulates the proliferation and apoptosis of gastric cancer cells by competitively binding to hsa-miR-656.
Key words: gastric cancer, circular RNA, proliferation, apoptosis, microRNA
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