摘要： 目的 探讨miR-149-5p对骨肉瘤（OS）细胞迁移能力的影响。方法 通过转染技术构建miR-149-5p过表达细胞系，采用PCR检测OS细胞中miR-149-5p的表达水平，采用Western blotting检测高迁移率族蛋白A2 （HMGA2）的表达，采用CCK-8实验检测不同处理因素下OS细胞的迁移能力。通过生物信息学网站starbase结合预测miR-149-5p与HMGA2 mRNA的结合位点。结果 与正常细胞相比，OS细胞中miR-149-5p呈低表达趋势（P<0.05），HMGA2呈高表达趋势（P<0.05）。miR-149-5p抑制细胞迁移，并可被HMGA2过表达所恢复。生物信息学网站证明HMGA2 mRNA存在与miR-149-5p的结合位点。结论 miR-149-5p通过HMGA2调控OS细胞的迁移。

miR-149-5p通过HMGA2调控骨肉瘤细胞迁移

朱英会¹, 仲伟俍², 王志坚¹, 仲涛¹, 田康²

1. 大连医科大学附属大连市第三人民医院骨科, 辽宁 大连 116033;
2. 大连医科大学附属第一医院骨科, 辽宁 大连 116011

收稿日期:2020-12-07出版日期:2021-06-30发布日期:2021-05-28
通讯作者:田康E-mail:dmu-tiankang@outlook.com
作者简介:朱英会(1981-),男,副主任医师,硕士.
基金资助:辽宁省高等学校科学研究一般项目（L2014360）


关键词: miR-149-5p, 高迁移率族蛋白A2, 迁移, 骨肉瘤
Abstract: Objective To explore the effect of miR-149-5p on the migration of osteosarcoma cells. Methods The expression of miR-149-5p in osteosarcoma cells was detected using PCR. An miR-149-5p-overexpressing cell line was constructed using transfection technology. The expression of miR-149-5p was detected using PCR,and the expression of high mobility group protein A2 (HMGA2) was detected through western blotting. The migration of cells under different conditions was detected using the Transwell assay. The binding sites of miR-149-5p and the HMGA2 mRNA were predicted through the bioinformatic website StarBase. Results Compared to normal cells,osteosarcoma cells exhibited a lower expression of miR-149-5p (P<0.05) and higher expression of HMGA2 (P<0.05). miR-149-5p inhibited cell migration,which could be restored by HMGA2 overexpression. The bioinformatic data revealed that the HMGA2 mRNA can bind to miR-149-5p. Conclusion miR-149-5p regulates the migration of osteosarcoma cells through HMGA2.
Key words: miR-149-5p, high mobility group protein A2, migration, osteosarcoma
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