摘要： 目的 探讨非小细胞肺癌进展过程中miR-203对ABCE1表达的调节作用。方法 选取20例非小细胞肺癌患者，采用实时PCR技术检测肺癌组织及对应癌旁组织内miR-203及ABCE1的表达及相关性。在非小细胞肺癌细胞内，采用脂质体转染上调miR-203，观察对ABCE1基因的表达及迁移能力和凋亡的影响。结果 癌组织内miR-203水平低于癌旁组织，ABCE1水平高于癌旁组织；上调非小细胞肺癌细胞内miR-203的表达后，ABCE1的表达降低，并抑制细胞的迁移，诱导凋亡。结论 在非小细胞肺癌中，miR-203能够抑制ABCE1基因的表达，进而抑制肺癌细胞的迁移并诱导凋亡。

非小细胞肺癌进展过程中miR-203对ABCE1的调节作用

周镝, 田野, 杨雪鹰

中国医科大学附属第四医院胸外科, 沈阳 110032

收稿日期:2020-11-11出版日期:2021-06-30发布日期:2021-05-28
通讯作者:杨雪鹰E-mail:yangxueying1974@163.com
作者简介:周镝(1989-),男,医师,硕士.
基金资助:辽宁省高等学校基本科研项目（LFWK201713）


关键词: miR-203, ABCE1, 非小细胞肺癌
Abstract: Objective To investigate the regulatory effect of miR-203 on ABCE1 expression in the progression of non-small cell lung cancer (NSCLC). Methods Twenty NSCLC patients were selected and the expression and correlation of miR-203 and ABCE1 in lung cancer tissues and corresponding adjacent tissues were detected by qRT-PCR. In NSCLC cells,miR-203 was up-regulated by liposome transfection to observe the gene expression of ABCE1 and the effect on cell migration and apoptosis. Results Expression of miR-203 in cancer tissues was lower than that in adjacent tissues,and ABCE1 expression in cancer tissues was correspondingly higher. ABCE1 expression was down-regulated after the up-regulation of miR-203 expression. Down-regulation of ABCE1 inhibited migration and induced apoptosis in NSCLC cells. Conclusion miR-203 can inhibit the migration and induce apoptosis of lung cancer cells by suppressing expression of ABCE1 in NSCLC.
Key words: miR-203, ABCE1, non-small cell lung cancer
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