摘要： 目的 探讨虾青素对过氧化氢（H₂O₂）诱导的骨髓间充质干细胞（BMSC）氧化应激的保护作用及可能机制。方法 体外培养人BMSC，采用H₂O₂建立氧化应激模型，应用不同浓度的虾青素预保护BMSC。CCK-8法检测细胞存活率，流式细胞术检测细胞凋亡率，生物化学法测定细胞中谷胱甘肽（GSH）含量、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量。Western blotting检测细胞中核因子E2相关因子2（Nrf2）、血红素加氧酶-1（HO-1）、还原型辅酶/醌氧化还原酶1（NQO1）蛋白含量。结果 虾青素明显提高H₂O₂诱导的氧化应激BMSC的存活率，提高细胞内SOD活性、GSH含量，降低细胞内MDA含量，降低细胞凋亡率，提高Nrf2蛋白表达量。结论 虾青素可能通过激活Nrf2/抗氧化响应元件通路，减轻H₂O₂诱导的BMSC氧化应激。

虾青素对过氧化氢诱导的骨髓间充质干细胞氧化应激的保护作用

李露壮¹, 姜衍¹, 徐爱华¹, 辛昊², 王京杨³, 孙永新¹

1. 中国医科大学附属第一医院康复医学科, 沈阳 110001;
2. 中国医科大学附属第一医院药学部, 沈阳 110001;
3. 中国医科大学口腔医学专业102期, 沈阳 110122

收稿日期:2020-11-13出版日期:2021-02-28发布日期:2021-01-21
通讯作者:孙永新E-mail:sunyxjp@hotmail.com
作者简介:李露壮(1996-),女,硕士研究生.
基金资助:国家自然科学基金（81572221）；沈阳市科技计划（20-205-4-092）


关键词: 虾青素, 氧化应激, 骨髓间充质干细胞, 核因子E2相关因子2
Abstract: Objective To investigate the effect of astaxanthin on hydrogen peroxide (H₂O₂)-induced oxidative stress response and the possible mechanism of that response in bone marrow mesenchymal stem cells (BMSCs). Methods Human BMSCs were cultured in vitro. H₂O₂ was used to induce oxidative stress in BMSCs. BMSCs were pretreated with varying concentrations of astaxanthin. Cell survival was analyzed via a CCK-8 assay,and cell apoptosis was detected using flow cytometry. A biochemical method was used to measure glutathione and malonyldialdehyde levels as well as superoxide dismutase activity. Western blotting was employed to determine nuclear factor erythroid-2-related factor 2 (Nrf2),heme oxygenase 1,and quinone oxidoreductase 1 expression levels. Results Astaxanthin significantly improved the survival rate of BMSCs that had been subjected to H₂O₂-induced oxidative stress;it also increased superoxide dismutase activity,increased glutathione level,and reduced malondialdehyde level. Furthermore,astaxanthin lowered the cell apoptosis rate and induced Nrf2 protein expression. Conclusion Astaxanthin may attenuate H₂O₂-induced oxidative stress in BMSCs by activating the Nrf2/antioxidant response element pathway.
Key words: astaxanthin, oxidative stress, bone marrow mesenchymal stem cell, nuclear factor erythroid-2-related factor 2
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