摘要： 目的 探讨马齿苋多糖对幼年糖尿病大鼠糖脂代谢、肾功能的影响及其可能的作用机制。方法 将60只4周龄SPF级雄性SD大鼠随机分为对照组（Con组）、糖尿病模型组（Mod组）以及马齿苋多糖组（PP组），每组20只。Con组采用普通饲料喂养，Mod组和PP组采用高脂饮食联合链脲佐菌素建立2型糖尿病大鼠模型。建模成功后，PP组采用马齿苋多糖［200 mg/（kg·d）］灌胃，Mod组及Con组给予等体积生理盐水灌胃，连续干预6周。各组大鼠进行血糖、血脂、肾功能相关指标测定，HE及PAS染色观察肾结构及形态，免疫组化和Western blotting测定肾转化生长因子β1（TGF-β1）、转运蛋白Smad3和结缔组织生长因子（CTGF）蛋白表达。结果 与Con组比较，Mod组甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDLC）、肾指数、尿素氮、肌酐和24 h尿蛋白水平显著升高，肾脏组织TGF-β1、Smad3和CTGF蛋白的表达显著上调，差异均有统计学意义（均P < 0.05）；与Mod组比较，PP组TG、TC、LDLC、空腹血糖、肾指数、尿素氮、肌酐和24 h尿蛋白水平显著降低，肾脏组织TGF-β1、Smad3和CTGF蛋白的表达显著下调，差异均有统计学意义（均P < 0.05）。结论 马齿苋多糖能够改善幼年糖尿病大鼠的糖脂代谢，降低尿蛋白水平，并且对幼年糖尿病大鼠肾功能起保护作用，其机制可能与TGF-β1/Smad3信号通路有关。

马齿苋多糖对幼年糖尿病大鼠糖脂代谢、肾功能的影响及其作用机制

王成祥¹, 刘玉霞², 常绍鸿¹, 邢二庆¹, 谢遂亮¹

1. 新乡市中心医院儿科, 河南 新乡 453000;
2. 新乡医学院第四临床学院儿科, 河南 新乡 453000

收稿日期:2020-06-15出版日期:2021-01-30发布日期:2021-01-06
通讯作者:刘玉霞E-mail:1060324653@qq.com
作者简介:王成祥(1973-),男,副主任医师,硕士.
基金资助:河南省医学科技攻关计划（172102310132）


关键词: 马齿苋多糖, 幼年糖尿病大鼠, 糖脂代谢, 肾功能, TGF-β1/Smad3信号通路
Abstract: Objective To observe the effects of Portulaca oleracea L. polysaccharides on glycolipid metabolism,renal function,and trasnforming growth factor β1(TGF-β1)/Smad3 signaling in juvenile diabetic rats,and to investigate the possible underlying mechanisms. Methods Sixty SPF male SD rats aged 4 weeks were randomly divided into three groups:control (Con) group,diabetes model (Mod) group,and Portulaca oleracea L. polysaccharide (PP) group. The Con group was fed a normal diet,while the Mod and PP groups were fed a high-fat diet combined with streptozotocin. After diabetes was successfully induced,diabetic model rats in the PP group were gavaged with 200 mg/(kg·d) of Portulaca oleracea L. polysaccharide for 6 weeks,and the Mod and Con groups were administered an equal volume of normal saline for the same period. The blood glucose and lipid levels and renal function of rats in each group were measured,and the renal structure and morphology were assessed via HE and PAS staining. The expression levels of renal TGF-β1,transporter Smad3,and connective tissue growth factor (CTGF) were determined by immunohistochemistry and western blotting. Results Triglycerides (TG),total cholesterol (TC),low-density lipoprotein cholesterol (LDLC),kidney weight index (KWI),urea nitrogen,creatinine,and 24 h urine protein levels were significantly increased in the Mod group compared to the Con group,and TGF-β1,Smad3,and CTGF protein expression in renal tissues were significantly upregulated (all P < 0.05). Compared with the Mod group,levels of TG,TC,LDLC,fasting blood glucose,KWI,urea nitrogen,creatinine,and 24 h urinary protein were significantly lower in the PP group,and TGF-β1,Smad3,and CTGF protein expression in renal tissues were significantly decreased (all P < 0.05). Conclusion Portulaca oleracea L. polysaccharides can improve glucose and lipid metabolism in juvenile diabetic rats and also reduce urinary protein levels and protect renal function in these animals. The underlying mechanism may be related to the TGF-β1/Smad3 signaling pathway.
Key words: Portulaca oleracea L. polysaccharide, juvenile diabetic rats, glycolipid metabolism, renal function, trasnforming growth factor β1/Smad3 signaling pathway
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