摘要： 目的 采用网络药理学方法筛选当归-菟丝子的活性成分，预测其治疗多囊卵巢综合征（PCOS）的潜在作用靶标和信号通路，进一步探究其作用机制。方法 通过中药系统药理学数据库与分析平台（TCMSP）筛选当归-菟丝子的活性成分和候选靶标，利用GeneCards数据库和基因疾病关联数据库（DisGeNET）去重、合并，预测PCOS的相关靶标，筛选两味中药的活性成分治疗疾病的潜在作用靶标。应用Cytoscape软件构建“疾病-药物-成分-靶点”网络。借助蛋白质相互作用数据库（STRING）构建潜在靶标蛋白相互作用网络，并找到作用的关键基因。使用功能注释生物信息学分析平台（DAVID），对当归-菟丝子治疗PCOS的潜在作用靶标进行生物学功能和代谢通路分析。结果 当归-菟丝子中共筛选出11种有效活性成分，根据靶点预测技术预测出相关靶点135个，主要包括丝氨酸/苏氨酸激酶1（AKT1）、丝裂原活化蛋白激酶1（MAPK1）、白细胞介素-6（IL-6）和雌激素受体1（ESR1）等，主要涉及糖尿病并发症AGE-RAGE信号通路、液体剪切应力、动脉粥样硬化和内分泌抵抗等信号通路。结论 本研究采用网络药理学方法初步预测了当归-菟丝子治疗PCOS的可能机制，为后续进一步研究提供思路。

基于网络药理学探讨当归-菟丝子治疗多囊卵巢综合征的作用靶标和信号通路

王翠翠, 马晓欣

中国医科大学附属盛京医院妇产科, 沈阳 110022

收稿日期:2019-12-25出版日期:2021-01-30发布日期:2021-01-06
通讯作者:马晓欣E-mail:maxiaoxin666@aliyun.com
作者简介:王翠翠(1986-),女,主治医师,博士.



关键词: 当归, 菟丝子, 多囊卵巢综合征, 网络药理学
Abstract: Objective To screen the active ingredients of Angelicae Sinensis Radix and Cuscutae Semen and predict the targets and signaling pathways they are involved in,using network pharmacology,thus exploring their potential mechanism in the treatment of polycystic ovary syndrome (PCOS). Methods All the active ingredients and targets of Angelicae Sinensis Radix-Cuscutae Semen were screened out from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The disease targets in PCOS were determined using the Genecards and DisGeNET databases. The potential targets of the active ingredients were screened using a Venn diagram. Cytoscape software was used to establish the "disease-drug-active ingredients-targets" network. The STRING database was used to construct the protein interaction network of drug-paired targets used for treating PCOS. Lastly,analysis of the biological functions and metabolic pathways of the potential targets was conducted using the Database for Annotation,Visualization,and Integrated Discovery (DAVID). Results A total of 11 effective and active ingredients were screened and 135 targets were predicted. The key target proteins for treating PCOS included AKT1,MAPK1,IL-6,and ESR1,among others. The pathways and mechanisms involved include the AGE-RAGE signaling pathway in diabetic complications,fluid shear stress,atherosclerosis,and endocrine resistance. Conclusion Based on the network pharmacology approach,this study is an initial prediction of the major targets and pathways of Angelicae Sinensis Radix-Cuscutae Semen in the treatment of PCOS,providing a direction for further studies.
Key words: Angelicae Sinensis Radix, Cuscutae Semen, polycystic ovary syndrome, network pharmacology
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