摘要： 目的 探讨泛素特异性蛋白酶22（USP22）在原发性中枢神经系统淋巴瘤（PCNSL）预后中的作用。方法 收集48例PCNSL患者脑组织，同时收集27例脑挫裂伤患者挫伤灶脑组织。采用实时荧光定量PCR（qRT-PCR）和Western blotting检测USP22 mRNA和蛋白的表达情况，并采用Kaplan-Meier法和Cox模型探讨USP22对PCNSL患者预后的影响。结果 qRT-PCR和Western blotting的结果显示，PCNSL组织中USP22 mRNA和蛋白的表达水平均高于挫伤灶脑组织（P<0.05）。随着USP22蛋白表达水平升高，肿瘤的大小和数量也相应增加。生存分析结果显示，USP22蛋白表达水平越高，患者预后越差（P<0.05）。结论 USP22高表达与PCNSL的不良预后密切相关，今后可作为治疗的靶点以改善PCNSL患者的预后。

泛素特异性蛋白酶22与原发性中枢神经系统淋巴瘤预后的关系

郑杰¹, 刘英丽², 康进生³, 崔雪花¹, 桑琳霞¹, 李文玲¹

1. 河北医科大学第二医院神经外科, 石家庄 050000;
2. 河北医科大学公共卫生学院流行病与卫生统计学教研室, 石家庄 050017;
3. 河北省人民医院神经外二科, 石家庄 050051

收稿日期:2020-03-31出版日期:2020-12-30发布日期:2020-12-09
通讯作者:李文玲E-mail:158626623@qq.com
作者简介:郑杰(1983-),男,主治医师,硕士.
基金资助:河北省医学科学研究重点课题（20180110）


关键词: 原发性中枢神经系统淋巴瘤, 泛素特异性蛋白酶22, 预后
Abstract: Objective To explore the effects of the ubiquitin-specific protease 22 (USP22) in patients with primary central nervous system lymphoma (PCNSL). Methods Brain tissues from 48 patients with PCNSL and 27 patients with cerebral contusion were collected. The expression of USP22 mRNA and protein were measured using quantitative real-time PCR (qRT-PCR) and western blotting,and the effects of USP22 on the prognosis of PCNSL were explored using Kaplan-Meier method and Cox model. Results The expression of USP22 mRNA and protein in PCNSL tissues was higher than that in the cerebral tissues with contusion (P<0.05). The expression of USP22 was identified to be correlated with tumor size and the number of lesions,and the prognosis of PCNSL in patients with high USP22 expression was poor (P<0.05). Conclusion The high expression of USP22 is closely related to the poor prognosis of PCNSL,and USP22 is a potential therapeutic target.
Key words: primary central nervous system lymphoma, ubiquitin-specific protease 22, prognosis
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