摘要： 目的 探讨微小（miRNA）结合位点f101基因的3’端非翻译区的单核苷酸多态性（SNP）对非霍奇金淋巴瘤（NHL）患者癌症风险和临床预后的影响。方法 miRNA结合位点f101基因的3’端非翻译区rs4901706 SNP基因分型采用连接酶检测反应。rs4901706与NHL生存相关性采用log-rank检验及Kaplan-Meier方法。多因素生存分析采用Cox比例风险模型。结果 携带rs4901706 AA基因型患者比携带AG/GG基因型患者生存时间长（P=0.017）。多因素分析结果表明，rs4901706 SNP与NHL患者总生存率独立相关（相对风险：1.768，95% CI：1.045~2.997，P=0.035）。rs4901706 SNP与NHL生存相关性在T细胞淋巴瘤亚型也得到验证（P=0.025）。结论 f101基因3’端非翻译区miRNA结合位点SNP可能影响NHL患者的生存，对临床预后有预测意义。

f101基因3’端非翻译区微小RNA结合位点单核苷酸多态性与非霍奇金淋巴瘤患者预后的关系

赵桂敏, 刁兰萍, 刘丽宏, 吴晓琳, 高哲, 高玉环

河北医科大学第四医院血液科, 石家庄 050000

收稿日期:2020-03-04出版日期:2020-12-30发布日期:2020-12-09
通讯作者:高玉环E-mail:ydsygao@sina.com
作者简介:赵桂敏(1978-),男,副教授,硕士.
基金资助:河北省科技支撑计划（135200）


关键词: 微小RNA, 单核苷酸多态性, rs4901706, 非霍奇金淋巴瘤, 预后
Abstract: Objective The microRNA (miRNA) binding-site single nucleotide polymorphism (SNP) rs4901706 located in the 3'-untranslated region (UTR) of f101 was genotyped in non-Hodgkin lymphoma (NHL) patients to assess their relationship with cancer risk and overall survival. Methods The miRNA binding site SNP of rs4901706 in the 3'-UTR of f101 was genotyped using the ligation detection reaction method. The association of rs4901706 in f101 with NHL survival was calculated with the log-rank test using the Kaplan-Meier method. Multivariate survival analysis was performed using a Cox proportional hazards model. Results Patients with the rs4901706 AA genotype presented a significantly longer survival time than patients with the AG/GG genotype (P=0.017). In addition,the results of the multivariate analysis revealed that the rs4901706 SNP was independently associated with the survival of NHL patients (RR:1.768,95% CI:1.045-2.997,P=0.035). Furthermore,the statistically significant association between the rs4901706 SNP and NHL survival was corroborated in the T-cell lymphoma (TCL) subtype of NHL (P=0.025). Conclusion Our study proved that the SNP at the miRNA binding site in the f101 3'-UTR is influential in determining the survival rate of patients with NHL. Thus,it may have a promising clinical prognostic and survival significance.
Key words: microRNA, single nucleotide polymorphism, rs4901706, non-Hodgkin lymphoma, prognosis
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