摘要： 目的 探讨雌激素对小鼠胰岛β细胞（Min6细胞）线粒体自噬的影响。方法 体外培养Min6细胞，将细胞分为对照组（正常Min6细胞）和雌激素（E2）组。通过透射电镜观察线粒体自噬体；采用Western blotting技术检测线粒体自噬相关蛋白Beclin-1、HSP60、TOM20和LC3的表达水平。若Beclin-1和LC3Ⅱ/LC3Ⅰ的表达水平增加、线粒体基质蛋白HSP60和线粒体膜蛋白TOM20的表达水平降低，则提示线粒体自噬激活。结果 与对照组相比，E2组Min6细胞中线粒体自噬体减少，Beclin-1和LC3Ⅱ/LC3Ⅰ的表达水平降低，HSP60和TOM20的表达水平增加。结论 雌激素对Min6细胞的线粒体自噬过程起抑制作用。

雌激素对Min6细胞线粒体自噬的影响

李思怡, 白博文, 张媚, 王雨佳, 周鑫宇, 赵玉岩

中国医科大学附属第一医院内分泌与代谢病科, 沈阳 110001

收稿日期:2020-02-21出版日期:2020-11-30发布日期:2020-10-31
通讯作者:赵玉岩E-mail:zhaoyybox@163.com
作者简介:李思怡(1993-),女,硕士研究生.
基金资助:国家自然科学基金（81470998）


关键词: 雌激素, Min6细胞, 线粒体自噬
Abstract: Objective To investigate the effects of estrogen on mitochondrial autophagy in mouse islet β cells(Min6 cells). Methods Min6 cells were cultured in vitro and divided into two groups:control group(normal Min6 cells)and estrogen(E2)-treated group. The mitochondrial autophagosomes were observed using transmission electron microscopy. The expression levels of mitochondrial autophagyrelated proteins Beclin-1,HSP60,TOM20,and LC3 were detected using Western blotting. Increased expression of Beclin-1 and LC3Ⅱ/LC3Ⅰ and decreased expression of the mitochondrial matrix protein HSP60 and mitochondrial membrane protein TOM20 indicate the activation of mitochondrial autophagy. Results Formation of mitochondrial autophagosomes decreased,expression of TOM20 and HSP60 increased,and expression of Beclin-1 and LC3Ⅱ/LC3Ⅰ decreased in E2-treated Min6 cells compared with those in the control group. Conclusion Estrogen inhibits mitochondrial autophagy in Min6 cells.
Key words: estrogen, Min6 cells, mitochondrial autophagy
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