摘要： 目的 探讨MYCT1通过PRSS21对喉癌细胞增殖和迁移的影响。方法 应用实时PCR和Western blotting方法检测喉癌中PRSS21的表达水平及MYCT1对PRSS21表达水平的影响；应用CCK-8实验和克隆形成实验检测MYCT1通过PRSS21对喉癌细胞增殖和克隆形成能力的影响；应用划痕实验检测MYCT1通过PRSS21对喉癌细胞迁移能力的影响。结果 PRSS21在喉癌中高表达（P < 0.05）；与对照组相比，MYCT1显著降低PRSS21在喉癌细胞中mRNA和蛋白质的表达水平（P < 0.05）；MYCT1通过PRSS21显著抑制喉癌细胞的增殖和克隆形成（P < 0.05）；MYCT1通过PRSS21显著抑制喉癌细胞的迁移能力（P < 0.05）。结论 MYCT1可通过抑制PRSS21的表达抑制喉癌细胞的增殖和迁移。

MYCT1通过PRSS21抑制喉癌细胞的增殖和迁移

陈芸, 孙媛媛, 富伟能

中国医科大学生命科学学院医学遗传学教研室, 沈阳 110122

收稿日期:2019-12-05出版日期:2020-10-30发布日期:2020-10-13
通讯作者:富伟能E-mail:wnfu@cmu.edu.cn
作者简介:陈芸(1995-),女,技师,硕士.
基金资助:国家自然科学基金（81372876）；辽宁省重点研发计划（2017225021）；辽宁省高等学校基本科研项目（LQNK201726）


关键词: MYCT1, PRSS21, 喉癌, 增殖, 迁移
Abstract: Objective To investigate the effects of MYCT1 on the PRSS21-mediated proliferation and migration of laryngeal carcinoma cells. Methods Real-time PCR and Western blotting were used to detect PRSS21 mRNA and protein expression,respectively in the context of MYCT1 silencing or overexpression in laryngeal carcinoma. The CCK-8 and clone formation assays were applied to monitor the effects of MYCT1 on the PRSS21-mediated proliferation and survival of laryngeal cancer cells. A scratch assay was further conducted to monitor the effect of MYCT1 on the migration (PRSS21-mediated) of laryngeal cancer cells. Results PRSS21 was highly expressed in laryngeal carcinoma (P < 0.05). Compared to controls,MYCT1 significantly inhibited the expression of PRSS21 both at mRNA and protein levels in laryngeal cancer cells (P < 0.05). Importantly MYCT1 significantly inhibited the proliferation,clone formation,and migration of laryngeal carcinoma cells,in a PRSS21 dependent fashion (P < 0.05). Conclusion MYCT1 represses the proliferation and migration of laryngeal carcinoma cells via the inhibition of PRSS21 expression.
Key words: MYCT1, PRSS21, laryngeal carcinoma, proliferation, migration
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