摘要： 目的 探讨不同氨基聚糖——透明质酸（HA）和硫酸软骨素（CS）对巨噬细胞极化的作用，旨在选出能减少炎症反应及组织损伤的细胞移植支架材料。方法 将小鼠骨髓源性巨噬细胞分别培养于HA/CS水凝胶中，在不同时间（0、4、24、48、72 h）收集标本，实时qPCR检测白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、诱导性一氧化氮合酶（iNOS）和精氨酸酶-1（Arg-1）表达水平。结果 与0 h比较，48 h HA水凝胶中巨噬细胞TNF-α和iNOS表达增加（P < 0.05）。与0 h比较，48 h CS水凝胶中巨噬细胞Arg-1表达增加（P < 0.05）。结论 HA可使巨噬细胞极化为具有促炎作用的M1型，而CS使巨噬细胞极化为具有抗炎作用的M2型。作为细胞移植支架材料，与HA比较，CS可以减少炎症损伤、保护组织。

不同氨基聚糖对巨噬细胞极化的影响

尹玉, 韩硕, 王国锋, 王琦, 金元哲

中国医科大学附属第四医院心内科, 沈阳 110032

收稿日期:2019-05-30出版日期:2020-10-30发布日期:2020-10-13
通讯作者:金元哲E-mail:jyzzhj@hotmail.com
作者简介:尹玉(1981-),女,主治医师,硕士.



关键词: 氨基聚糖, 透明质酸, 硫酸软骨素, 巨噬细胞极化, 影响
Abstract: Objective We examined the effects of different glycosaminoglycans (hyaluronic acid and chondroitin sulfate) on macrophage polarization,thinking on the design of a better hydrogel scaffold to attenuate tissue inflammation and injury. Methods We cultured bone marrow derived macrophage in hyaluronic acid/chondroitin sulfate-containing hydrogel for 0,4,24,48,and 72 h,and then conducted RT-qPCR to evaluate the expression of interleukin-1β (IL-1β),tumor necrosis factor-α (TNF-α),inducible nitric oxide synthase (iNOS),and arginase-1 (Arg-1). Results Comparing with the 0 h time-point,the mRNA expression of TNF-α and iNOS increased from the 48 h time-point onwards in the context of the hyaluronic acid hydrogel (P < 0.05);the mRNA expression of Arg-1 also increased after 48 h in the context of the chondroitin sulfate hydrogel (P < 0.05). Conclusion Hyaluronic acid probably polarizes macrophages into an M1 phenotype,promotes inflammation,while chondroitin sulfate polarizes macrophages into an anti-inflammatory M2 phenotype. Therefore,chondroitin sulfate is a better scaffold material than hyaluronic acid to suppress inflammation and attenuate injury.
Key words: glycosaminoglycan, hyaluronic acid, chondroitin sulfate, macrophage polarization, effect
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