摘要： 目的 采用葡聚糖硫酸钠（DSS）诱导建立小鼠急性结肠炎模型，探讨芦丁对急性结肠炎是否具有保护作用以及是否通过调控肠固有层淋巴细胞（LPL）中辅助性T细胞（Th）应答发挥作用。方法 采用3% DSS诱导建立小鼠急性结肠炎模型，实验组以芦丁50 mg·kg^-1·d^-1剂量灌胃，对照组以等体积生理盐水灌胃。计算小鼠疾病活动指数，评估结肠病理组织学得分。应用流式细胞术检测肠LPL中Th1和Th17的比例和绝对数。结果 与对照组相比，实验组小鼠体质量下降更慢，疾病活动指数降低，结肠炎症较轻，组织学评分显著降低，肠LPL中CD4⁺和CD8⁺T淋巴细胞比例显著减少，CD4⁺T细胞中γ干扰素（IFN-γ）⁺细胞比例、白细胞介素（IL）-17A⁺细胞比例和绝对数以及IFN-γ⁺IL-17A⁺细胞绝对数均明显减少。结论 芦丁通过抑制小鼠肠固有层Th1和Th17细胞应答，在DSS诱导的急性实验性结肠炎病变形成中发挥重要保护性作用。

芦丁在葡聚糖硫酸钠诱导的小鼠急性结肠炎中的作用及其机制

褚旭芳¹, 王笑楠¹, 范秋灵², 汪旭¹

1. 中国医科大学附属第一医院 消化内科, 沈阳 110001;
2. 中国医科大学附属第一医院 肾内科, 沈阳 110001

收稿日期:2019-06-03出版日期:2020-09-30发布日期:2020-09-15
通讯作者:汪旭E-mail:wangxu_cmu@163.com
作者简介:褚旭芳(1993-),女,硕士研究生.
基金资助:国家自然科学基金（81770724）


关键词: 芦丁, 葡聚糖硫酸钠诱导的结肠炎, 辅助性T细胞1, 辅助性T细胞17
Abstract: Objective To establish an acute colitis model in mice using dextran sulfate sodium (DSS),and to investigate the protective effect of rutin in acute colitis and its prospective role in regulating helper T cell response in the intestinal lamina propria lymphocytes (LPL). Methods A mouse model of acute colitis was set up,where 3% DSS was used to induce colitis. The experimental group and the control group were intragastrically administrated with rutin at a dose of 50 mg·kg^-1·d^-1 and equal volume of normal saline,respectively. The disease activity index was calculated,and the colon histopathology score was assessed. Flow cytometry was used to detect the proportion and absolute number of helper T cell 1 (Th1) and 17 (Th17) in the intestinal LPL. Results Compared with the control group,the experimental group had slower weight loss,a lower disease activity index,milder colonic inflammation,a significantly lower histological score,and a significantly lower proportion of CD4⁺ and CD8⁺ T lymphocytes in the intestinal LPL. The proportion of interferon-γ(IFN-γ)⁺ cells,proportion and absolute number of interleukin-17A (IL-17A)⁺ cells,and absolute number of IFN-γ⁺ IL-17A⁺ cells were significantly reduced in CD4⁺ T cells. Conclusion Rutin plays an important protective role in the pathogenesis of DSS-induced acute colitis by inhibiting the Th1 and Th17 cell responses in the intestinal lamina propria of mice.
Key words: rutin, dextran sulfate sodium-induced colitis, helper T cell 1, helper T cell 17
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