摘要： 目的 探讨长链非编码RNA（lncRNA）RUNX1-IT1对口腔鳞状细胞癌（OSCC）增殖、迁移的影响及其作用机制。方法 利用实时qPCR检测lncRNA RUNX1-IT1在OSCC组织和细胞系中的表达水平。采用CCK8、流式细胞术以及Transwell实验检测OSCC细胞生物学行为。利用Western blotting检测上皮-间质转化（EMT）过程相关的蛋白表达。结果 lncRNA RUNX1-IT1在OSCC组织和细胞系中表达水平与癌旁正常组织（P<0.001）和正常角质细胞（P<0.01）比较显著增高。lncRNA RUNX1-IT1表达水平和淋巴结转移相关（P<0.05）。过表达或沉默lncRNA RUNX1-IT1后可以调节E-cadherin、N-cadherin、β-catenin、Vimentin蛋白水平；也显著上调或下调细胞增殖迁移相关的蛋白（PCNA、Cyclin D1、MMP2）水平。结论 lncRNA RUNX1-IT1在OSCC中高表达，且可能通过EMT过程调控OSCC细胞增殖、迁移。

长链非编码RNA RUNX1-IT1对口腔鳞状细胞癌增殖、迁移的影响及其作用机制

王雪¹, 李琳², 张红¹, 张雪², 王振华¹

1. 中国医科大学 生命科学学院生理学教研室, 沈阳 110122;
2. 中国医科大学 附属口腔医院干诊科, 沈阳 110002

收稿日期:2019-09-12出版日期:2020-09-30发布日期:2020-09-15
通讯作者:王振华E-mail:wzhcmusl@163.com
作者简介:王雪(1993-),女,硕士研究生.
基金资助:辽宁省自然科学基金（2015020750，20180550782）


关键词: 长链非编码RNA RUNX1-IT1, 口腔鳞状细胞癌, 增殖, 迁移, 上皮-间质转化
Abstract: Objective To understand the effect of long non-coding RNA (lncRNA) RUNX1-IT1 on proliferation and migration of oral squamous cell carcinoma (OSCC). Methods RT-qPCR was performed to evaluate the relative expression of lncRNA RUNX1-IT1 in OSCC tissues and cells. CCK8,flow cytometry and Transwell assays were performed to detect the biological functions of the OSCC cell line. Western blotting was used to measure the proteins associated with epithelial-mesenchymal transition (EMT). Results lncRNA RUNX1-IT1 expression levels were higher in OSCC tissues and cells,than those observed in adjacent normal tissues (P<0.001) and normal keratinocytes (P<0.01). Expression of lncRNA RUNX1-IT1 was significantly associated with lymph node metastasis (P<0.05). Overexpression and silencing of lncRNA RUNX1-IT1 could regulate the proteins levels of E-cadherin,N-cadherin,β-catenin,Vimentin. The expression levels of proteins involved in proliferation and migration (PCNA,Cyclin D1,and MMP2) were also upregulated or downregulated. Conclusion lncRNA RUNX1-IT1 expression levels are elevated in OSCC. We propose that lncRNA RUNX1-IT1 regulates cell proliferation and migration through EMT.
Key words: long non-coding RNA RUNX1-IT1, oral squamous cell carcinoma, proliferation, migration, epithelial-mesenchymal transition
PDF全文下载地址:

https://journal.cmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=2599