摘要： 目的 探究氟伐他汀钠对皮肤鳞状细胞癌细胞增殖、凋亡及PI3K/AKT/mTOR通路的影响。方法 体外培养SCL-1细胞，MTT法检测氟伐他汀钠对SCL-1细胞增殖的影响；流式细胞术检测细胞凋亡的变化；实时qPCR法检测PI3K、Akt、mTOR mRNA相对表达量。结果 不同浓度氟伐他汀钠处理SCL-1细胞后，以浓度依赖性和时间依赖性的方式抑制SCL-1细胞增殖（P<0.01）。流式细胞术显示不同浓度氟伐他汀钠干预SCL-1细胞24 h、48 h后早期凋亡率较对照组明显升高（P<0.01），且具有浓度依赖性。不同浓度氟伐他汀钠培养SCL-1细胞24 h后PI3K、Akt、mTOR mRNA相对表达量随药物浓度的增加而降低，与对照组比较有统计学差异（P<0.01）。结论 氟伐他汀钠可抑制SCL-1细胞增殖，诱导SCL-1细胞凋亡，其作用机制可能与抑制PI3K/Akt/mTOR通路有关。

氟伐他汀钠对皮肤鳞状细胞癌细胞增殖、凋亡及PI3K/Akt/mTOR通路的影响

刘鸿雁, 孙冲, 李倩, 刘梅

中国医科大学附属第一医院皮肤科, 沈阳 110001

收稿日期:2019-02-27出版日期:2020-09-30发布日期:2020-09-15
通讯作者:刘梅E-mail:15040396633@139.com
作者简介:刘鸿雁(1992-),女,硕士研究生.



关键词: 皮肤鳞状细胞癌, 氟伐他汀钠, 细胞增殖, 细胞凋亡, PI3K/Akt/mTOR通路
Abstract: Objective To investigate the effects of fluvastatin sodium on the proliferation,apoptosis,and PI3K/Akt/mTOR pathway in a human cutaneous squamous cell carcinoma cell line,SCL-1. Methods The MTT assay was used to detect the effect of fluvastatin sodium on the proliferation of SCL-1 cells. Flow cytometry was used to detect the apoptotic changes in SCL-1 cells after treatment with fluvastatin sodium. mRNA expression levels of PI3K,Akt,and mTOR in the cells treated with different concentrations of fluvastatin sodium were detected using RT-qPCR. Results Proliferation of SCL-1 cells was inhibited by different doses of fluvastatin sodium in concentration- and time-dependent manners (P<0.01).Flow cytometry revealed that the early apoptotic rate of SCL-1 cells treated with different concentrations of fluvastatin sodium was significantly increased after 24-h and 48-h interventions compared with the control group (P<0.01),and the apoptotic rate showed concentration dependence. The mRNA expression levels of PI3K,Akt,and mTOR in SCL-1 cells treated with different concentrations of fluvastatin sodium decreased with increase in the concentration of fluvastatin sodium at 24 h,which was statistically significant compared with the control group (P<0.01). Conclusion Fluvastatin sodium inhibits proliferation and induces apoptosis of SCL-1 cells by inhibiting the PI3K/Akt/mTOR signaling pathway.
Key words: cutaneous squamous cell carcinoma, fluvastatin sodium, cell proliferation, apoptosis, PI3K/Akt/mTOR pathway
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