摘要： 目的 调查SULT4A1基因rs549267654、rs2285162、rs963263和ss2137544034位点在中国北方汉族群体的多态性分布，评价其法医学应用价值。方法 采用Sanger测序及PCR-RFLP技术对226例中国北方汉族健康无关个体样品进行检测，应用Haploview4.2进行数据的统计分析。结果 rs549267654、rs2285162和rs963263位点的基因型分布符合Hardy-Weinberg平衡，个人识别力分别为0.649、0.619和0.647，非父排除率分别为0.187、0.177和0.187。同时，发现1个新的6碱基插入/缺失位点ss2137544034。结论 rs549267654、rs2285162和rs963263位点在中国北方汉族人群中具有较好的遗传多态性，可用于法医学个人识别与亲权鉴定。

SULT4A1基因4个位点在中国北方汉族群体中的分布及法医学意义

吴军胜, 丁梅, 雍梦馨, 赵梦阳, 邢佳鑫, 王保捷

中国医科大学法医学院法医物证教研室, 沈阳 110122

收稿日期:2019-04-26出版日期:2020-09-30发布日期:2020-09-15
通讯作者:王保捷E-mail:wangbj77@163.com
作者简介:吴军胜(1993-),男,硕士研究生.



关键词: 法医学, 多态性, 单核苷酸多态性, SULT4A1, 汉族
Abstract: Objective To investigate the distribution of polymorphisms in the rs549267654,rs2285162,rs963263,and ss2137544034 loci of the SULT4A1 gene in the Han population of northern China and to evaluate their application value in forensic medicine. Methods Sanger sequencing and PCR-RFLP were used to detect polymorphisms in SULT4A1 in 226 healthy individuals from northern China. The data were analyzed using Haploview 4.2. Results The genotype distributions of rs549267654,rs2285162,and rs963263 were in accordance with Hardy-Weinberg equilibrium;their discrimination powers were 0.649,0.619,and 0.647,respectively,and the probabilities of paternity were 0.187,0.177,and 0.187,respectively. Concomitantly,a new 6-base insertion/deletion site,ss2137544034,was identified. Conclusion rs549267654,rs2285162,and rs963263 exhibit high genetic polymorphism in the Han population of northern China and can be used for forensic personal identification as well as paternity identification.
Key words: forensic genetics, polymorphism, single nucleotide polymorphisms, SULT 4A1, Han population
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