摘要： 目的 研究大鼠慢性肝损伤中线粒体功能障碍及能量异常。方法 给予大鼠皮下注射50%四氯化碳大豆油溶液，制备慢性肝损伤模型，测定肝组织细胞线粒体膜电位、膜肿胀度、细胞内辅酶ⅠNAD（H）含量、三磷酸腺苷（ATP）含量。结果 与对照组相比，模型组大鼠肝细胞线粒体膜电位水平、膜肿胀度水平、辅酶ⅠNAD（H）、ATP水平明显降低（P < 0.05），随着造模时间的延长，损伤程度逐渐增加（P < 0.01）。结论 在四氯化碳致大鼠慢性肝损伤模型中，肝脏线粒体功能受损，能量代谢异常，提示肝脏线粒体功能障碍所致能量代谢异常在临床肝脏疾病中具有重要意义。

大鼠慢性肝损伤的线粒体功能障碍及其影响

张靓睿, 王诗琪, 王红昀, 董若瑶, 孟胜男

中国医科大学药学院药剂学教研室, 沈阳 110122

收稿日期:2019-11-26出版日期:2020-08-30发布日期:2020-08-04
通讯作者:孟胜男E-mail:shnmeng@hotmail.com
作者简介:张靓睿(1995-),女,硕士研究生.
基金资助:辽宁省高等学校基本科研项目重点项目（LZDK201705）


关键词: 四氯化碳, 慢性肝损伤, 线粒体损伤, 膜电位
Abstract: Objective To study mitochondrial dysfunction and energy abnormality in chronic liver injury in rats. Methods The chronic liver injury model was prepared by subcutaneous injection of 50% carbon tetrachloride soybean oil solution. Mitochondrial membrane potential,membrane swelling,intracellular coenzymeⅠNAD (H) content,and adenosine triphosphate (ATP) content were determined. Results Compared with the control group,the mitochondrial membrane potential level,membrane swelling degree,ATP,and coenzyme ⅠNAD (H) levels in the model group of rats were significantly decreased (all P < 0.05). The degree of injury gradually and significantly increased with time (P < 0.01). Conclusion Chronic liver injury was induced using carbon tetrachloride in rats. The observations of impaired liver mitochondrial function and abnormal energy metabolism indicate that the abnormal energy metabolism caused by liver mitochondrial dysfunction is important in diseases featuring clinical liver injury.
Key words: carbon tetrachloride, chronic liver injury, mitochondrial damage, membrane p
PDF全文下载地址:

https://journal.cmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=2584