摘要： 目的 探讨果糖二磷酸醛缩酶A（ALDOA）在不同肺癌亚型中的预后预测价值及其相关通路。方法 从癌症基因组图谱（TCGA）数据库中下载肺癌患者ALDOA的表达谱及临床信息资料，分析ALDOA表达在肺癌不同病理亚型中与临床病理学参数的相关性及对预后的影响。采用基因集富集分析（GSEA）预测ALDOA的相关通路。结果 ALDOA的表达在肺癌病理亚型中存在差异（P < 0.05），且在肺腺癌患者中ALDOA高表达与患者预后不良显著相关（P < 0.05）。肺腺癌中ALDOA表达水平与肿瘤浸润深度（T分期，P=0.026）、淋巴结转移数量（N分期，P=0.026）相关。ALDOA高表达样本富集到糖酵解、低氧通路、氧化磷酸化等代谢相关通路以及G₂M检查点、凋亡、上皮-间质转化等肿瘤增殖及转移相关通路。结论 肺腺癌中ALDOA高表达是预后不良因素，ALDOA表达水平可作为判断患者是否发生转移及预测预后的潜在标志物。

利用TCGA数据分析果糖二磷酸醛缩酶A对肺癌预后的预测效果

王一喆¹, 金悦², 王晓楠¹, 包博文², 程阳¹, 车晓芳², 胡雪君¹

1. 中国医科大学附属第一医院老年呼吸感染科, 沈阳 110001;
2. 中国医科大学附属第一医院肿瘤内科, 辽宁省抗肿瘤药物与生物治疗重点实验室, 沈阳 110001

收稿日期:2019-05-09出版日期:2020-07-30发布日期:2020-07-02
通讯作者:胡雪君E-mail:huxuejun2015@126.com
作者简介:王一喆(1994-),女,医师,博士研究生.
基金资助:国家自然科学基金（81472193）；辽宁省重点研发指导计划资源平台建设项目（2018225060）


关键词: 肺腺癌, 果糖二磷酸醛缩酶A, 癌症基因组图谱, 预后
Abstract: Objective To investigate the prognostic value and related pathways of fructose-bisphosphate aldolase A (ALDOA) in different pathological subtypes of lung cancer. Methods The expression profiles and clinical data of ALDOA in lung cancer patients were downloaded from the cancer genome atlas (TCGA) database. The correlation between ALDOA expression and the clinical pathological parameters of lung cancer,and prognosis in different pathological subtypes were analyzed. Related pathways of key genes were predicted by gene set enrichment analysis. Results The expression level of ALDOA varied among the pathological subtypes of lung cancer (P < 0.05), and its high expression in patients with lung adenocarcinoma was significantly associated with poor prognosis (P < 0.05). The expression level of ALDOA in lung adenocarcinoma was related only to the depth of tumor invasion (T stage,P=0.026) and the number of lymph node metastasis (N stage,P=0.026). Samples exhibiting high expression of ALDOA were enriched in metabolic pathways such as glycolysis, hypoxia pathway,and oxidative phosphorylation,as well as tumor proliferation and metastasis related pathways such as G₂M checkpoints, apoptosis,and epithelial-mesenchymal transition. Conclusion High expression of ALDOA in lung adenocarcinoma is a factor associated with poor prognosis,and the expression level of ALDOA can be used as a potential marker for metastasis and prognosis.
Key words: lung adenocarcinoma, fructose-diphosphate aldolase A, the cancer genome atlas, prognosis
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