摘要： 目的 通过研究阿托伐他汀对大鼠心肌梗死（AMI）后促血管生成素1（Ang-1）和Tie2 mRNA表达的影响，探讨阿托伐他汀对于AMI后心肌血管生成的作用机制。方法 80只SD雄性大鼠随机分为正常（CG）组、单纯心肌梗死（AG）组和心肌梗死联合阿托伐他汀（AAG）组；AAG组于术后第3天开始以2.5 mg阿托伐他汀溶液灌胃给药，AG组及CG组以同等剂量生理盐水灌胃；各组分别于术后14 d及28 d取材，实时PCR技术检测心肌Ang-1及Tie2 mRNA的表达。结果 在AMI建模14 d时AAG组与AG组Tie2 mRNA的表达水平有统计学差异（P < 0.05）；14 d时Ang-1 mRNA的表达水平3组差异无统计学意义（P > 0.05）；在AMI模型28 d时3组Ang-1 mRNA及Tie2 mRNA表达比较，差异无统计学意义（P > 0.05）。结论 阿托伐他汀能够促进AMI血管生成，其可能通过上调Tie2 mRNA的表达，促进新生血管的形成。

阿托伐他汀对急性心肌梗死大鼠Ang-1及其受体Tie2 mRNA表达的影响

陈仕毅¹, 付玉平^2,3, 冯晓晶¹, 刘少奎¹

1. 大连大学附属中山医院循环科, 辽宁 大连 116001;
2. 中国医科大学附属第一医院骨科, 沈阳 110001;
3. 北部战区总医院和平院区骨科, 沈阳 110042

收稿日期:2020-01-06出版日期:2020-07-30发布日期:2020-07-02
通讯作者:刘少奎E-mail:dlliushaokui@sina.com
作者简介:陈仕毅(1981-),男,主治医师,硕士.
基金资助:辽宁省自然科学基金（2017011058-301）；辽宁省大连市卫生局课题（1611098）


关键词: 阿托伐他汀, 心肌梗死, Ang-1, Tie2
Abstract: Objective In this article,we analyzed the effect of atorvastatin treatment on the mRNA expression levels of Ang-1 and its receptor Tie2 after acute myocardial infarction (AMI) in rats and investigated the mechanism of atrovastatin activity on myocardial angiogenesis after AMI. Methods Here,80 male Sprague-Dawley (SD) rats were randomly divided into three groups:the normal group; the intervention group,which was treated with atorvastatin;and the non-drug treated intervention group. The AMI intervention group was initially administered a dose of 2.5 mg atorvastatin in solution via gavage feeding.As controls,the non-drug administered AMI group and the normal control group rats were administered with the same dosage of normal saline. Samples from all the groups were collected on 14 and 28 days after operation. The mRNA expression levels of Ang-1 and Tie2 in myocardial tissues were detected by RT-PCR. Results After 14 days of feeding,the mRNA expression levels of Tie2 in the AMI atorvastatin intervention group was significantly higher than those in the normal mice group (P < 0.05). There were no significant differences among the expression levels of Ang-1 mRNA in the intervention group,the non-drug treatment group,and the normal group (P > 0.05). Moreover,at day 28,there were no significant differences in the mRNA expression levels of Tie2 in all the three groups of rats (P > 0.05). Conclusion Atorvastatin treatment increases the expression of Tie2 mRNA and promotes the formation of new blood vessels.
Key words: atorvastatin, myocardial infarction, Ang-1, Tie2
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