摘要： 目的 探讨长链非编码RNA（lncRNA）lnc-ZNF37A-2（RP11-672F9.1，AL133271.1）对肺癌细胞迁移和侵袭能力的影响。方法 利用GEPIA网站分析lnc-ZNF37A-2在癌症基因组图谱（TCGA）数据库不同分期肺癌组织中的表达，同时通过starBase v3.0分析lnc-ZNF37A-2表达量与肺癌患者总生存期的相关性。通过Co-LncRNA网站预测与lnc-ZNF37A-2相关的mRNA，进行基因富集和通路富集分析。干扰肺癌细胞中lnc-ZNF37A-2的表达，采用实时定量PCR检测lnc-ZNF37A-2的表达效率。采用划痕实验、Transwell侵袭实验检测细胞的迁移、侵袭能力。结果 lnc-ZNF37A-2在Ⅳ期肺癌患者中的表达高于Ⅰ~Ⅲ期患者，且lnc-ZNF37A-2高表达与总生存期呈负相关；lnc-ZNF37A-2相关的mRNA基因富集分析得到的通路与细胞黏附相关；沉默lnc-ZNF37A-2后肺癌细胞A549的迁移和侵袭能力明显降低。结论 lnc-ZNF37A-2在肺癌细胞中起到癌基因的作用，与lnc-ZNF37A-2共表达的mRNA影响了肿瘤微环境中细胞黏附和细胞外基质受体的相互作用，沉默lnc-ZNF37A-2抑制了肺癌细胞迁移和侵袭能力。

长链非编码RNA lnc-ZNF37A-2对肺癌细胞迁移和侵袭能力的影响

周发忱¹, 舒鑫¹, 周欣²

1. 大连医科大学附属第一医院胸外科, 辽宁 大连 116011;
2. 大连医科大学基础医学院组织胚胎学教研室, 辽宁 大连 116044

收稿日期:2020-04-18出版日期:2020-07-30发布日期:2020-07-02
通讯作者:周欣E-mail:dl_zhoufch@126.com
作者简介:周发忱(1977-),男,副主任医师,博士研究生.
基金资助:辽宁省自然科学基金（20180551083）


关键词: 长链非编码RNA, lnc-ZNF37A-2, 肺癌, 侵袭, 迁移
Abstract: Objective To investigate the effects of long non-coding RNA (lncRNA) lnc-ZNF37A-2 (RP11-672F9.1,AL133271.1) on the invasion and migration abilities of lung cancer cells. Methods GEPIA website was used to analyze lnc-ZNF37A-2 expression at different pathological stages in lung cancer samples,using the data acquired from The Cancer Genome Atlas (TCGA) database. The relationship between lnc-ZNF37A-2 expression and overall survival (OS) of lung cancer patients was assessed using the starBase v3.0 program. Geneset and pathway enrichment analyses were conducted to evaluate the potential functions of mRNAs co-expressed with lnc-ZNF37A-2 that were predicted using the Co-LncRNA program. We used real-time quantitative PCR to assess silencing efficiency after performing lnc-ZNF37A-2 silencing in the lung cancer cells. Wound healing and Transwell invasion assays were performed to assess migration and invasion abilities of the cancer cells. Results lnc-ZNF37A-2 expression was significantly increased in stage Ⅳ lung cancer patients than in stage Ⅰ to Ⅲ lung cancer patients. Survival analysis revealed that increased lnc-ZNF37A-2 expression might be related to decreased OS in lung cancer patients. Gene enrichment analysis of lnc-ZNF37A-2-related mRNAs indicated that lnc-ZNF37A-2 majorly affects genes involved in cell adhesion. Moreover,silencing of lnc-ZNF37A-2 reduced the migration and invasion abilities of lung cancer cells. Conclusion lnc-ZNF37A-2 plays a crucial role in regulating the expression of oncogenes in lung cancer cells. The mRNAs co-expressed with lnc-ZNF37A-2 affect cell adhesion and extracellular matrix receptor interaction in tumor microenvironment. Furthermore,silencing of lnc-ZNF37A-2 leads to the inhibition of migration and invasion of lung cancer cells.
Key words: long non-coding RNA, lnc-ZNF37A-2, lung cancer, invasion, migration
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