摘要： 目的 研究hMOF表达下调对子宫内膜癌Ishikawa细胞增殖、凋亡、迁移和侵袭的影响。方法 hMOF小干扰RNA（siRNAhMOF）转染子宫内膜癌细胞系Ishikawa，筛选和鉴定转染效率，并将细胞分为对照组、空白转染对照组（siRNA-NC组）和实验组（siRNA-hMOF组）。采用MTT法测定细胞生长曲线，流式细胞术检测细胞凋亡，划痕实验检测细胞迁移，Transwell实验检测细胞侵袭。结果 siRNA-hMOF转染子宫内膜癌Ishikawa细胞后，与siRNA-NC组相比，siRNA-hMOF组细胞的增殖速度减慢，凋亡率增加，迁移和侵袭能力下降。结论 干扰hMOF表达可抑制子宫内膜癌Ishikawa细胞的增殖、迁移和侵袭，促进细胞的凋亡；hMOF在子宫内膜癌的恶性生长转移中发挥重要作用。

hMOF对子宫内膜癌细胞生物学行为的影响

齐跃, 叶秋霖, 刘娟娟, 林蓓

中国医科大学附属盛京医院妇产科, 沈阳 110004

收稿日期:2020-03-23出版日期:2020-07-30发布日期:2020-07-02
通讯作者:林蓓E-mail:linbei88@hotmail.com
作者简介:齐跃(1980-),女,主治医师,博士.
基金资助:盛京自由研究者计划（201804）


关键词: hMOF, 子宫内膜癌, 增殖, 凋亡, 迁移, 侵袭
Abstract: Objective To study the effect of downregulated hMOF expression on the proliferation,apoptosis,migration,and invasion of the endometrial cancer cell line Ishikawa. Methods siRNA of hMOF was transfected into the endometrial cancer cell line Ishikawa, and the transfection efficiency was screened and identified. The cells were divided into control,siRNA-NC,and siRNA-hMOF groups. We performed MTT assay to detect the cell proliferation,Annexin-Ⅴ-APC/7AAD double staining to detect the rate of apoptosis,wound healing test to detect the migration ability,and Transwell test to detect the invasion ability of the cells. Results Compared with the siRNA-NC group,the proliferation,migration,and invasive capacity of cells decreased while apoptosis increased in the siRNA-hMOF group after transfection with siRNA-hMOF. Conclusion Interference with hMOF expression can inhibit the proliferation,migration,and invasion of the endometrial cancer cell line Ishikawa and promote the apoptosis. Thus,hMOF plays an important role in the malignant growth and metastasis of endometrial cancer.
Key words: hMOF, endometrial cancer, proliferation, apoptosis, migration, invasion
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