摘要： 目的 基于细胞焦亡（pyroptosis）理论研究白细胞介素（IL）-1β与IL-18在抗结核药物性肝损伤（ADLI）中的表达及临床意义。方法 选取2016年1月至2017年1月于唐山市第四医院接受住院治疗的肺结核初治患者，收集符合ADLI的41患者为病例组，按照年龄、性别、用药方案匹配的原则选取用药后非ADLI（non-ADLI）患者41例为对照组。酶联免疫法（ELISA）检测ADLI组和非ADLI组患者血清IL-1β及IL-18水平，用受试者工作特征曲线下面积（ROC-AUC）评估IL-1β预测ADLI的价值。结果 2组患者血清IL-1β水平差异有统计学意义（P<0.05），IL-18水平无统计学差异（P>0.05）；IL-1β与天冬氨酸转移酶（AST）、丙氨酸氨基转移酶（ALT）及碱性磷酸酶（ALP）存在正相关（均P<0.05），IL-1β水平预测ADLI的AUC为0.889。结论 IL-1β可能是与ADLI发生发展相关的分子标记物，可以成为ADLI诊断的一个新作用靶点。

基于细胞焦亡理论研究白细胞介素-1β与白细胞介素-18在抗结核药物性肝损伤中的临床意义

李玉红¹, 郝明媛², 焦连丽², 王爱欣², 任琦¹, 冯福民¹

1. 华北理工大学公共卫生学院河北省煤矿卫生与安全重点实验室, 河北 唐山 063210;
2. 呼伦贝尔市传染病医院检验科, 内蒙古 呼伦贝尔 162650

收稿日期:2018-10-11出版日期:2020-05-30发布日期:2020-05-13
通讯作者:冯福民E-mail:fm_feng@sina.com.cn
作者简介:李玉红(1989-),女,博士研究生.
基金资助:国家自然科学基金（81670525）


关键词: 抗结核药物性肝损伤, 细胞焦亡, 白细胞介素-1β, 白细胞介素-18
Abstract: Objective To investigate the expression and clinical significance of interleukin(IL)-1β and IL-18 in anti-tuberculosis druginduced liver injury(ADLI) based on the theory of pyroptosis. Methods Patients with pulmonary tuberculosis who were hospitalized in the Fourth Hospital of Tangshan between January 2016 and January 2017,inclusive,were enrolled. The case group comprised 41 patients with ADLI,and 41 patients without ADLI,matched for age,gender,and medication regimen,were selected as the control group. The levels of serum IL-1β and IL-18 in the case and control groups were measured using enzyme-linked immunosorbent assay(ELISA). The area under the IL-1β in predicting ADLI. Results There was a significant difference in serum IL-1β levels between the two groups(P<0.05), but the IL-18 levels were not significantly different for the two groups(P>0.05). There were positive correlations between IL-1β and AST, ALT,and ALP(all P<0.05),and the AUC of IL-1β level that predicted ADLI was 0.889. Conclusion IL-1β may be a molecular marker related to the development of ADLI and may be a new target for the diagnosis of ADLI.
Key words: anti-tuberculosis drug-induced liver injury, pyroptosis, human interleukin-1β, human interleukin-18
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