摘要： 目的 讨论M2型巨噬细胞相关因子精氨酸酶Ⅰ（Arg1）和STAT6在慢性重度牙周炎中的表达情况。方法 收集15例慢性重度牙周炎患牙的牙龈病损组织作为实验组，15例需要拔除第三磨牙的健康牙龈组织作为对照组，Western blotting检测Arg1和STAT6蛋白表达，实时PCR检测相关基因Arg1和STAT6 mRNA的表达情况。结果 实验组牙周病损组织中Arg1、STAT6蛋白和mRNA表达水平高于对照组，差异有统计学意义（P<0.01）。结论 在慢性重度牙周炎中M2型细胞介导免疫应答增高，提示M2型巨噬细胞参与慢性重度牙周炎的发病过程并发挥重要作用。

M2型巨噬细胞相关因子在慢性重度牙周炎中的表达

谭昭¹, 刘乙臻¹, 王琳源¹, 关宁², 高秀秋¹

1. 锦州医科大学 附属第二医院牙周黏膜科, 辽宁 锦州 121000;
2. 锦州医科大学 附属第一医院脑与脊髓损伤重点实验室, 辽宁 锦州 121001

收稿日期:2019-05-27出版日期:2020-05-30发布日期:2020-05-13
通讯作者:高秀秋E-mail:jzgaoxiuqiu1988@163.com
作者简介:谭昭(1991-),男,硕士研究生.
基金资助:国家自然科学基金青年基金（81600870）；辽西地区口腔微创临床治疗中心创新平台（2018201002）；辽宁省博士启动基金（201601360）


关键词: M2型巨噬细胞, 慢性重度牙周炎, 精氨酸酶Ⅰ, STAT6
Abstract: Objective To investigate the expression of the M2 macrophage-related factors arginase1(Arg1) and STAT6 in chronic severe periodontitis. Methods The gingival lesions of 15 patients with chronic severe periodontitis were collected and considered as one experimental group for this study. Fifteen healthy gingival tissues requiring removal of the third molar were used as the samples in the control group. The protein expression of Arg1 and STAT6 was analyzed by western blotting,and the mRNA expression of Arg1 and STAT6 was estimated using real-time PCR. Results Both the protein and mRNA expression levels of Arg1 and STAT6 in the lesions of chronic severe periodontitis were higher than those observed in the control group(P<0.01). Conclusion M2 cell-mediated immune response is elevated in chronic severe periodontitis,suggesting that M2 macrophages play an important role in the pathogenesis of chronic severe periodontitis.
Key words: M2 macrophage, chronic periodontitis, arginase1, STAT6
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