摘要： 目的 探讨敲减肝癌SMMC7721细胞中CCAAT/增强子结合蛋白β（C/EBPβ）对Myc target 1（MYCT1）的表达及肝癌细胞迁移能力的影响。方法 应用实时定量PCR分别检测敲减C/EBPβ和MYCT1的转染效率。应用实时定量PCR、Western blotting分别检测敲减C/EBPβ对MYCT1 mRNA及蛋白表达的影响。应用划痕实验及Transwell实验检测敲减C/EBPβ对MYCT1在细胞迁移方面的影响。结果 实时定量PCR结果显示，敲减MYCT1后，SMMC7721细胞MYCT1 mRNA表达水平显著下调；敲减C/EBPβ后，C/EBPβ mRNA表达水平显著下调，MYCT1 mRNA表达水平显著上调。Western blotting结果显示，敲减C/EBPβ后MYCT1蛋白表达水平显著上调。划痕实验结果及Transwell实验结果显示，敲减MYCT1组肝癌细胞较对照组迁移能力显著增强，敲减C/EBPβ组细胞较对照组细胞迁移能力显著下降；而共转染敲减C/EBPβ与敲减MYCT1后，SMMC7721细胞迁移能力显著恢复。结论 在肝癌细胞中，敲减C/EBPβ可通过显著上调MYCT1表达抑制细胞迁移能力。

C/EBPβ通过调控MYCT1表达影响肝癌细胞迁移

梁雪亭, 冯博

中国医科大学附属第一医院放射科介入病房, 沈阳 110001

收稿日期:2019-03-06出版日期:2020-05-30发布日期:2020-05-13
通讯作者:冯博E-mail:fb6772@sina.com
作者简介:梁雪亭(1992-),男,硕士研究生.
基金资助:辽宁省高等学校基本科研项目（LFWK201704）


关键词: CCAAT/增强子结合蛋白β, Myc target 1, 肝癌, 迁移
Abstract: Objective To investigate the effect of CCAAT/enhancer binding protein β (C/EBPβ) knock-down on Myc target 1(MYCT1) expression and hepatoma cell(SMMC7721) migration. Methods C/EBPβ and MYCT1 knock-down efficiency was detected by real-time quantitative polymerase chain reaction(RT-qPCR). RT-qPCR and Western blotting were used to detect the effect of C/EBPβ knock-down on MYCT1 mRNA expression and protein expression,respectively. Scratch assays and transwell assays were used to investigate the effect of C/EBPβ knock-down on hepatoma cell migration. Results RT-qPCR showed that MYCT1 knock-down significantly reduced MYCT1 expression. C/EBPβ knock-down significantly reduced C/EBPβ mRNA expression and increased MYCT1 expression. Western blotting showed that C/EBPβ knock-down significantly increased MYCT1 protein levels. Scratch and transwell assays showed that MYCT1 knockdown significantly enhanced cell migration compared to the control. Moreover,C/EBPβ knock-down significantly inhibited cell migration compared to the control. Knocking down both C/EBPβ and MYCT1 at once reversed cell migration. Conclusion C/EBPβ knock-down significantly up-regulated MYCT1 expression and might inhibit migration in hepatoma cells.
Key words: CCAAT/enhancer binding protein β, Myc target 1, hepatoma, migration
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