摘要： 目的 研究谷氨酸AMPA/Kainate受体拮抗剂CNQX对断尾诱发小鼠前扣带回皮质神经元c-fos基因表达的影响。方法 免疫组化方法分别研究尾静脉注射和蛛网膜下腔注射CNQX对尾末端2.5 cm剪断后0.5 h小鼠前扣带回皮质神经元c-fos基因表达的影响。结果 正常小鼠前扣带回皮质神经元有少量c-fos基因表达，而断尾后0.5 h小鼠前扣带回皮质神经元c-fos基因表达显著增强。同时，尾静脉注射和蛛网膜下腔注射CNQX均能拮抗该c-fos基因表达的显著增强。结论 断尾后小鼠前扣带回皮质神经元c-fos基因表达显著增强的过程有外周与中枢谷氨酸AMPA/Kainate受体参与。

CNQX拮抗断尾诱发小鼠前扣带回皮质神经元c-fos基因的表达

李娜然¹, 商丽宏¹, 杨宇², 姚阳², 吴敏范²

1. 沈阳医学院 机能与形态实验中心, 沈阳 110034;
2. 沈阳医学院 生理学教研室, 沈阳 110034

收稿日期:2019-03-01出版日期:2020-04-30发布日期:2020-04-18
通讯作者:吴敏范E-mail:minfanwu0594@sina.com.cn
作者简介:李娜然(1964-),女,高级实验师,本科.
基金资助:辽宁省科学技术计划（L2015020391）；沈阳医学院科学研究基金（20151009）


关键词: 幻肢痛, 前扣带回皮质, AMPA/Kainate受体, c-fos基因
Abstract: Objective We aimed to study the effect of glutamate AMPA/kainate receptor antagonist CNQX on tail amputation induced c-fos gene expression in the anterior cingulate cortex neurons of mice. Methods CNQX was injected into the caudal vein or subarachnoid space. The effect of CNQX on gene expression at 0.5 h after cutting 2.5 cm of the tail extremity was studied using immunohistochemistry. Results In the control group,a small amount of c-fos gene was expressed in the anterior cingulate cortex neurons. However,the expression of c-fos in the anterior cingulate cortex neurons of mice increased significantly 0.5 h after tail amputation;this increase was antagonized by CNQX injection into the caudal vein or subarachnoid space. Conclusion Glutamate peripheral and central AMPA/kainate receptors are involved in tail amputation induced increase in c-fos gene expression in the anterior cingulate cortex neurons of mice.
Key words: phantom limb pain, anterior cingulate cortex, AMPA/Kainate receptor, c-fos gene
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