摘要： 目的 观察miR-625-3p在卵巢浆液性癌中的表达，分析其与炎性细胞因子单核细胞趋化蛋白1（MCP-1）、间质巨噬细胞的数量及增殖指数的关系。方法 选取67例卵巢浆液性癌患者作为观察组，60例卵巢浆液性囊腺瘤患者作为对照组。应用实时荧光定量PCR法检测miR-625-3p的表达，应用免疫组化方法检测石蜡包埋组织中MCP-1和巨噬细胞标记物CD163的表达，应用Western blotting法检测新鲜标本中MCP-1和CD163的表达。结果 2组比较，miR-625-3p表达、MCP-1阳性率、巨噬细胞数量、MCP-1和CD163半定量表达的差异均有统计学意义（P<0.05）。观察组中，miR-625-3p的表达量、MCP-1和CD163的半定量表达在不同肿瘤最大径、病变级别、脉管癌栓、淋巴结转移、TNM分期中的差异有统计学意义（P<0.05）。观察组中miR-625-3p的表达与增殖指数、MCP-1、CD163的表达呈正相关。生存分析显示，miR-625-3p的表达与预后有关。结论 卵巢浆液性癌中miR-625-3p高表达，对促进病变的形成有一定作用，miR-625-3p可能对调控细胞增殖、巨噬细胞形成及炎性细胞因子生成有一定影响。术后检测miR-625-3p的表达，可能对判断预后有一定价值。

miR-625-3p在卵巢浆液性癌中的表达及意义

王莉, 蔡旺

锦州医科大学附属第一医院妇科, 辽宁 锦州 121000

收稿日期:2019-05-21出版日期:2020-02-29发布日期:2019-12-26
通讯作者:蔡旺E-mail:adacaiw@163.com
作者简介:王莉(1971-),女,副教授,硕士.
基金资助:辽宁省自然科学基金（20180551184）


关键词: 卵巢浆液性癌, miR-625-3p, 单核细胞趋化蛋白1, CD163, 巨噬细胞, 预后
Abstract: Objective To observe the expression of miR-625-3p in ovarian serous carcinoma and analyze its relationship with the inflammatory factors monocyte chemoattractant protein-1(MCP-1),number of interstitial macrophages,and the cell proliferation index. Methods Patients with ovarian serous carcinoma(n=67)were selected as the observation group and those with ovarian serous cystadenoma(n=60)as the control group. The expression of miR-625-3p was detected by real-time PCR. The expression of MCP-1 and macrophage marker CD163 in paraffin-embedded tissues was detected by immunohistochemistry. The expression of MCP-1 and CD163 in fresh specimens was detected by Western blotting. Results There were significant differences in the expression of miR-625-3p,positive rate of MCP-1,number of macrophages and semiquantitative expression of MCP-1 and CD163 between the two groups(P<0.05). In the observation group,the expression of miR-625-3p,MCP-1 and CD163 differed significantly between tumors with different maximum diameter,pathological grade,vascular tumor thrombus,lymph node metastasis,and TNM stage(P<0.05). The expression of miR-625-3p was positively correlated with proliferation index,MCP-1,and CD163. Survival analysis showed that the expression of miR-625-3p was related to prognosis. Conclusion The high expression of miR-625-3p may promote pathological changes in ovarian serous carcinoma. miR-625-3p may be involved in the regulation of cell proliferation,macrophage formation,and inflammatory cytokine production. Detection of miR-625-3p expression after operation may have prognostic value.
Key words: ovarian serous carcinoma, miR-625-3p, monocyte chemoattractant protein-1, CD163, macrophages, prognosis
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