摘要： 目的 探讨槐耳清膏对人肾小管上皮细胞（HK-2细胞）内质网应激的保护作用。方法 将HK-2细胞分为对照组（正常HK-2细胞），毒胡萝卜素（TG）组（5 μmol/L TG处理8 h），5 mg/mL、10 mg/mL中药组（5、10 mg/mL槐耳清膏处理12 h），中药+TG组（5、10 mg/mL槐耳清膏处理4 h后加入5 μmol/L TG共同作用8 h）。采用Western blotting检测各组细胞内质网源性转录因子（CHOP）和内质网分子伴侣葡萄糖调节蛋白78（GRP78）的表达情况。结果 与对照组相比，TG组细胞CHOP和GRP78的表达显著增加（P<0.05），中药组CHOP及GRP78表达无明显变化（P>0.05）。与TG组相比，中药（5、10 mg/mL）+TG组CHOP与GRP78表达显著减少。结论 TG可诱导人肾小管上皮HK-2细胞发生内质网应激反应。适宜浓度的槐耳清膏可能通过下调CHOP、GRP78表达，抑制HK-2细胞内质网应激反应，从而发挥保护肾小管上皮细胞的作用。

槐耳清膏对人肾小管上皮细胞内质网应激损伤的保护作用

傅桐¹, 侯玲¹, 范洋², 杜悦¹

1. 中国医科大学附属盛京医院 小儿肾脏风湿免疫科, 沈阳 110004;
2. 中国医科大学附属盛京医院 研发中心, 辽宁 本溪 117000

收稿日期:2019-01-14出版日期:2020-02-29发布日期:2019-12-26
通讯作者:杜悦E-mail:duy@sj-hospital.org
作者简介:傅桐(1993-),男,医师,硕士研究生.
基金资助:辽宁省自然科学基金（2015020492）


关键词: 槐耳清膏, 人肾小管上皮细胞, 内质网应激
Abstract: Objective To investigate the protective effect of the Chinese herbal medicine Huaierqing on human renal tubular epithelial cells(HK-2 cells)with endoplasmic reticulum stress(ERS). Methods The cells were divided into the following six groups:control(normal HK-2 cells),thapsigargin(TG),(HK-2 cells treated with 5 μmol/L TG for 8 h),5 or 10 mg/mL Chinese herbal medicine(HK-2 cells treated with 5 or 10 mg/mL Chinese herbal medicine Huaierqing for 12 h),and 5 or 10 mg/mL Chinese herbal medicine +TG(HK-2 cells treated with 5 or 10 mg/mL Chinese herbal medicine Huaierqing for 4 h,followed by 5 μmol/L TG treatment as a coefficient reaction for 8 h). Western blotting analysis was used to measure the expression of glucose-regulated protein(GRP)78 and C/-EBP homologous protein(CHOP). Results Compared with the control group,CHOP and GRP78 were markedly overexpressed in the TG group(P<0.05);however,they were not expressed significantly in both the Chinese herbal medicine groups(P>0.05). Compared with the TG group,CHOP and GRP78 expression levels were significantly downregulated in the 5 or 10 mg/mL Chinese herbal medicine +TG groups(P<0.05). Conclusion TG induced ERS in HK-2 cells. At the appropriate concentration,the Chinese herbal medicine Huaierqing exhibited a protective effect by reducing the expression of CHOP and GRP78,which inhibited ERS in HK-2 cells.
Key words: Chinese herb medicine Huaierqing, endoplasmic reticulum stress, human renal tubular epithelial cell
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