摘要： 目的 研究红景天提取物对大鼠慢性束缚应激所致焦虑障碍的作用及其机制。方法 将40只雄性SD大鼠随机分为5组，即非束缚对照组，束缚模型组（大鼠束缚器束缚3 h/d，连续21 d），红景天低剂量治疗组（束缚应激开始前30 min灌胃给予红景天提取物80 mg·kg^-1·d^-1），红景天高剂量（240 mg·kg^-1·d^-1）治疗组和红景天高剂量对照组。在最后1次束缚结束24 h后，依次用旷场（OF）实验和高架十字迷宫（EPM）实验观察大鼠焦虑样行为。行为学观察结束后，立即处死大鼠，取海马组织，检测海马谷氨酸浓度，用实时定量PCR检测海马区脑源性神经营养因子（BDNF）表达水平。结果 OF实验中，束缚模型组大鼠自发活动总路程、中央区活动路程和运动时间明显缩短，但治疗组以上改变均有改善。EPM实验中，束缚模型组大鼠进入开放臂的次数和开放臂逗留时间均减少，而治疗组较束缚模型组明显改善，且开放臂停留时间呈红景天剂量依赖性。束缚模型组海马谷氨酸浓度明显增高，BDNF表达明显减少，而治疗组较束缚模型组明显改善。结论 红景天提取物可阻断慢性束缚应激所致大鼠焦虑样行为，作用机制可能与其降低海马谷氨酸系统过度兴奋和增强海马区BDNF系统功能密切相关。

红景天提取物对慢性束缚应激所致大鼠焦虑障碍的作用

王也夫^1,2, 王玉花², 吴桐², 李平², 崔光成²

1. 新乡医学院研究生院, 河南 新乡 453003;
2. 齐齐哈尔医学院精神卫生学院精神药理学教研室, 黑龙江 齐齐哈尔 161006

收稿日期:2019-07-09出版日期:2020-02-29发布日期:2019-12-26
通讯作者:崔光成E-mail:gccui@vip.tom.com
作者简介:王也夫(1993-),男,硕士研究生.
基金资助:黑龙江省自然科学基金（H201497，H2016100）；齐齐哈尔医学院科学院项目（QMSI2019Z-20）


关键词: 红景天, 焦虑, 谷氨酸, 脑源性神经营养因子
Abstract: Objective To evaluate the possible therapeutic effects of Rhodiola rosea L. extracts(RhrLE)on rats' chronic restraint stress-induced anxiety,and investigate its relevant mechanisms. Methods Restraint stress was established by placing male Sprague-Dawley rats in a restraint container for 3 h/d for 21 d. RhrLE(80 and 240 mg·kg^-1·d^-1)were intragastrically administered to the rats 30 min before every restraint stress exposure. Twenty-four hours after the final restraint stress episode,an open field(OF)test and elevated plus maze(EPM)test were conducted to examine the anxiety-like behaviors in rats. The glutamate levels and BDNF gene expression in the hippocampus were measured using a glutamate test kit analysis and a real-time polymerase chain reaction(qPCR)assay,respectively. Results In the OF test,the restraint stress significantly reduced the total distance traveled,distance traveled in the central zone, and duration of their stay in the central zone,which were all improved by RhrLE administration. In the EPM test,chronic restraint stress significantly decreased the number of entries into the open arms and duration of staying in the open arms,while RhrLE rescued these decreases;the effects of RhrLE on the latter showed a dose-dependent relationship. Meanwhile,in the kit test and qPCR assay,RhrLE normalized the increase in glutamate levels and reduced BDNF mRNA expression in the hippocampus. Conclusion Treatment with RhrLE can ameliorate chronic restraint stress-induced anxiety in rats,which may be mediated through improving glutamatergic transmission and brain-derived neurotrophic factor system function in the hippocampus.
Key words: Rhodiola rosea L., anxiety, glutamate, brain derived neurotrophic factor
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