张显
职称：副教授、硕士生导师
学历/学位：研究生、博士
电话：**
电子邮箱：zx@jiangnan.edu.cn


简介
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长期从事发酵微生物选育、工业微生物代谢工程育种、工业/食品用酶开发等研究，主要承担微生物学（含实验）、微生物遗传育种（含实验）课程的讲授工作。近年来在微生物发酵法生产四碳基平台化合物、生物催化法生产高值氨基酸、工业/食品用酶改良等方面开展多项工作。合作发表SCI论文30余篇，获授权国家发明专利20余项，美国专利3项。近5年来主持863计划子课题、国家自然科学基金、江苏省自然科学基金、中国博士后面上项目和特别资助项目5项。获2018江苏省科学技术奖二等奖（5/9）、2018中国商业联合会科技进步奖特等奖（3/14）、2016中国轻工业联合会科学技术发明二等奖（4/6）。



学习工作经历
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2003.09—2007.06 江南大学，生物科学专业，理学学士
2007.09—2014.01 江南大学，发酵工程专业，工学博士
2014.03—2016.08 江南大学，生物工程学院，讲师
2016.09—至 今 江南大学，生物工程学院，副教授
2017.06—至 今 江南大学，生物工程学院，硕士生导师
其中：
2018.10—2019.10 美国俄亥俄州立大学，化学与生化分子工程系，访问****



主要成果
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一、论文（论著）发表情况
合作发表SCI论文30余篇（其中第一作者在Metabolic Engineering，Amino Acids等国际权威期刊发表SCI论文10余篇，共同通讯作者SCI文章6篇，单篇最高IF值>8.0）。发表的代表性SCI文章（*通讯作者；Δ共同第一作者）
部分代表性论文如下：
1. Zhang Xian^Δ, Zhang Rongzhen^Δ, Bao Teng, Rao Zhiming*, Yang Taowei, Xu Meijuan, Xu Zhenghong, Li Huazhong*, Yang Shangtian. The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis [J]. Metabolic Engineering, 23: 34-41, 2014.
2. Zhang Xian, Rao Zhiming*, Li Jingjing, Zhou Junping, Yang Taowei, Xu Meijuan, Bao Teng, Zhao Xiaojing. Improving the acidic stability of Staphylococcus aureus α?acetolactate decarboxylase in Bacillus subtilis by changing basic residues to acidic residues [J]. Amino Acids, 47(4):707–717, 2015.
3. Song Liu, Xian Zhang*, Fei Liu, Meijuan Xu, Taowei Yang, Mengfei Long, Junping Zhou, Tolbert Osire, Shangtian Yang, Zhiming Rao*. Designing of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for Production of 1,2-Amino Alcohols from Epoxides. ACS Synthetic Biology. 2019, 8:734-743.
4. Xian Zhang, Rumeng Han, Teng Bao, Xiaojing Zhao, Xiangfei Li, Manchi Zhu, Taowei Yang, Meijuan Xu, Minglong Shao, Youxi Zhao, Zhiming Rao*. Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method. Microbial Cell Factories. 2019, 18:128.
5. Xian Zhang, Manchi Zhu, Rumeng Han, Youxi Zhao, Kewei Chen, Kai Qian, Minglong Shao, Taowei Yang, Meijuan Xu, Jianzhong Xu*, Zhiming Rao*. A novel 3-phytosterone-9α-hydroxylase oxidation subunit and itsapplication in bioconversion of 4-androstene-3,17-dione to9α-hydroxy-4-androstene-3,17-dione coupling with a NADH regeneration formatedehydrogenase. Molecules. 2019, 24(14):2534.
6. Zhang Xian^Δ, Bao Teng^Δ, Rao Zhiming*, Yang Taowei, Xu Zhenghong, Yang Shangtian, Li Huazhong*. Two-stage pH Control Strategy Based on The pH Preference of Acetoin Reductase Regulates Acetoin and 2,3-butanediol Distribution in Bacillus subtilis [J]. PLoS One, 9(3): e91187, 2014.
7. Zhang Xian, Zhang Rongzhen*, Bao Teng, Yang Taowei, Xu Meijuan, Li Huazhong, Xu Zhenghong, Rao Zhiming*. Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis [J]. J Ind Microbiol Biotechnol, 40(9):1067-1076, 2013.
8. Zhang Xian, Yang Taowei, Lin Qing, Xu Meijuan, Xia Haifeng, Xu Zhenghong, Li Huazhong, Rao Zhiming*. Isolation and identification of an acetoin high production bacterium that can reverse transform 2,3-butanediol to acetoin at the decline phase of fermentation[J]. World J Microbiol Biotechnol, 27(12):2785-2790, 2011.
9. Zhang Xian, Zhang Rongzhen*, Yang Taowei, Zhang Jing, Xu Meijuan, Li Huazhong, Xu Zhenghong, Rao Zhiming*. Mutation breeding of acetoin high producing Bacillus subtilis blocked in 2,3-butanediol dehydrogenase [J]. World J Microbiol Biotechnol, 29: 1783-1789, 2013.
10. Xian Zhang, Zhiming* R, Zhang L, Meijuan X, Taowei Y. Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration. SpringerPlus 5:1207, 2016.
11. Xian Zhang, Dan Wu, Taowei Yang, Meijuan Xu, Zhiming Rao*. Over-expression of Mycobacterium neoaurum 3-ketosteroid-Δ1-dehydrogenase in Corynebacterium crenatum for efficient bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione. Electronic Journal of Biotechnology, 2016, 24: 84-90.
12. Teng Bao^Δ, Xian Zhang^Δ, Xiaojing Zhao, Zhiming Rao*, Taowei Yang，Shangtian Yang. Regulation of the NADH pool and NADH/NADPH ratio redistributes acetoin and 2,3-butanediol proportion in Bacillus subtilis. Biotechnology Journal, 8(10): 1298-1306, 2015.
13. Bao Teng^Δ, Zhang Xian^Δ, Rao Zhiming*, Zhao Xiaojing, Zhang Rongzhen, Yang Taowei, Xu Zhenghong, Yang Shangtian. Efficient whole-cell biocatalyst for acetoin production with NAD⁺ regeneration system through homologous co-expression of 2,3-butanediol dehydrogenase and NADH oxidase in engineered Bacillus subtilis. PLoS One, 9(7): e102951, 2014.
14. Xu Li, Xian Zhang*, Shuqin Xu, Meijuan Xu, Taowei Yang, Li Wang, Huiling Zhang, Haitian Fang, Tolbert Osire, Zhiming Rao*.Insight into the thermostability of thermophilic L-asparaginase and non-thermophilic L-asparaginase II through bioinformatics and structural analysis. Applied Microbiology and Biotechnology. 2019, 103(17):7055-7070
15. Xian Zhang, Zhaoyang Xu, Song Liu, Kai Qian, Meijuan Xu, Taowei Yang, Jianzhong Xu*, Zhiming Rao*. Improving the Production of Salt-Tolerant Glutaminase by Integrating Multiple Copies of Mglu into the Protease and 16S rDNA Genes of Bacillus subtilis 168. Molecules. 2019, 24(3):592.
16. Chunlin Tan, Xian Zhang*, Zhijing Zhu, Meijuan Xu, Taowei Yang, Tolbert Osire, Shangtian Yang, Zhiming Rao*. Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440. Microbial Cell Factories. 2019, 18(12):14.
17. Xu Li, Shuqin Xu, Xian Zhang*, Meijuan Xu, Taowei Yang, Li Wang, Huiling Zhang, Haitian Fang, Tolbert Osire, Shangtian Yang, Zhiming Rao*. Design of a high-efficiency synthetic system for l-asparaginase production in Bacillus subtilis. Engineering in Life Sciences. 2019, 19(3):229-239.
18. Samuel Niyomukiza, Bao Teng, Zhang Xian*, Yang Taowei, Xu Meijuan, Li Xin, Komera Irene, Philibert Tuyishime, Rao Zhiming*. Optimized whole cell biocatalyst fromacetoin to 2,3-butanediol through coexpression of acetoin reductase with NADH regeneration systems in engineered Bacillus subtilis. J Chem Technol Biotechnol, 2017, 92(9):2477-2487.
19. Xu Li, Xian Zhang*, Shuqin Xu, Hengwei Zhang, Meijuan Xu, Taowei Yang, Li Wang, Haifeng Qian, Huiling Zhang, Haitian Fang, Tolbert Osire, Zhiming Rao*, Shangtian Yang. Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases. Scientific Reports, 2018, 8:7915.
20. Minglong Shao, Xian Zhang, Zhiming Rao*, Meijuan Xu, Taowei Yang, Hui Li, Zhenghong Xu, Shangtian Yang. Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration.Green Chemistry, 2016, 18:1774-1784.
21. Rongzhen Zhang, Taowei Yang, Zhiming Rao*, Hongmei Sun, Meijuan Xu, Xian Zhang, Zhenghong Xu, Shangtian Yang. Efficient one-step Preparation of γ-Aminobutyric Acid from Glucose without Exogenous Cofactor by the Designed Corynebacterium glutamicum. Green Chemistry. 2014, 16: 4190-4197.
22. Man Zaiwei, Zhiming Rao*, Xu Meijuan, Guo Jing, Yang Taowei, Zhang Xian, Xu Zhenghong*. Improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Metabolic Engineering. 2016, 38:310.
23. Yang Taowei, Rao Zhiming*, Hu Guiyuan, Zhang Xian, Liu Mei, Dai Yue, Xu Meijuan, Xu Zhenghong, Yang Shangtian. Metabolic engineering of Bacillus subtilis for redistributing the carbon flux to 2,3-butanediol by manipulating NADH levels. Biotechnology for Biofuels, 8:129, 2015.
24. Taowei Yang, Zhiming Rao*, Xian Zhang, Meijuan Xu, Zhenghong Xu, Shang-Tian Yang. Metabolic engineering strategies for acetoin and 2,3-butanediol production: Advances and prospects, Critical Reviews in Biotechnology, 2017, 37(8): 990-1005.
二、专利情况
参与申报发明专利50余项（其中申报国际发明专利12项），获授权中国发明专利20余项。
部分代表性专利如下：
1. Zhiming Rao, Junxian Zheng, Meijuan Xu, Taowei Yang, Xian Zhang. Signal peptide, L-glutamic acid synthesized using konjac flour and methods of using same. United States Patent, No.: US ** B2, 2019.
2. Zhiming Rao, Zaiwei Man, Meijuan Xu, Taowei Yang, Xian Zhang. Method for increasing yield of L-arginine by knocking out flavin reductases. United States Patent, No.: US ** B2, 2019.
3. 张显，饶志明，杨套伟，徐美娟，韩如梦。一种稳定性提高的NADH氧化酶及其在乙偶姻生产中的应用。中国发明专利，授权号：6.X
4. 饶志明，张显，杨套伟，徐美娟，李静静，张静，刘俊佳。利用调控蛋白ALsR适度加强枯草芽孢杆菌乙偶姻合成。中国发明专利，授权号： ZL.0.
5. 饶志明，杨套伟，张显，徐美娟，满在伟。利用一株安全菌株发酵生物柴油副产物粗甘油生产2,3-丁二醇。中国发明专利，授权号：ZL7.2
6. 饶志明，杨套伟，张显，徐美娟，满在伟。通过加强辅酶循环再生速率提高Bacillus amyloliquefaciens 2，3-丁二醇产量。中国发明专利，授权号：ZL5.2
7. 饶志明，杨套伟，张显，徐美娟。一种微生物发酵选择性生产3-羟基-2-丁酮和2,3-丁二醇的方法。中国发明专利，授权号：ZL4.8
8. 饶志明, 李静静, 张显, 徐美娟, 杨套伟. 利用重组大肠杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶. 中国发明专利. 授权号： ZL0.0
三、承担教学科研项目情况
1. 国家自然科学基金-青年基金：Bacillus subtilis芽孢合成期乙偶姻合成代谢流重排研究（**），2016.1-2018.12，主持，22.6万元
2. “863”项目“有机酸生物制造”子课题：α-氨基丁酸合成多酶催化体系协同调控与修饰（2015AA021004-4），2015.1-2017.12，主持，40万元
3. 江苏省自然科学基金-青年基金：枯草芽孢杆菌细胞衰亡期乙偶姻合成调控研究（BK**），2015.7-2018.6，主持，20万元
4. 中国博士后第9批特别资助：枯草芽孢杆菌芽孢形成期乙偶姻合成调控及分子机制研究（2016T90421），2016.1-2017.12，主持，15万元
5. 中国博士后第57批面上一等资助（2015M570407）：L-天冬酰胺酶热稳定性改造及分子机理研究，2015.1-2016.12，主持，8万元
四、获奖情况（含指导学生获奖）
1. 2016中国轻工业联合会科学技术发明奖，二等奖，(个人排名4/6)
2. 2018江苏省科学技术奖，二等奖，(个人排名5/9)
3. 2018中国商业联合会科技进步奖, 特等奖, (个人排名3/14)
4. 2018第二届全国大学生生命科学竞赛，一等奖，第一指导教师
5. 2019江南大学本科优秀毕业论文指导教师